Curated Information
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Home > Curated Information Page > PubMed Id: 21499261
Guarani V, et al. (2011) Acetylation-dependent regulation of endothelial Notch signalling by the SIRT1 deacetylase. Nature 473, 234-8 21499261
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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K1764-ac - Notch 1 (mouse)
Modsite: LWFPEGFkVSEASkk SwissProt Entrez-Gene
Orthologous residues
Notch 1 (human): K1774‑ac, Notch 1 (mouse): K1764‑ac, Notch 1 (rat): K1764‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEACETYLASE SIRT1 (human) activation of upstream enzyme, mutation in upstream enzyme recognition motif, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation modification-specific antibody
Downstream Regulation
Effect of modification (function):  protein stabilization, ubiquitination

K1770-ac - Notch 1 (mouse)
Modsite: FkVSEASkkkRREPL SwissProt Entrez-Gene
Orthologous residues
Notch 1 (human): K1780‑ac, Notch 1 (mouse): K1770‑ac, Notch 1 (rat): K1770‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEACETYLASE SIRT1 (human) activation of upstream enzyme, mutation in upstream enzyme recognition motif, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation modification-specific antibody
Downstream Regulation
Effect of modification (function):  protein stabilization, ubiquitination

K1771-ac - Notch 1 (mouse)
Modsite: kVSEASkkkRREPLG SwissProt Entrez-Gene
Orthologous residues
Notch 1 (human): K1781‑ac, Notch 1 (mouse): K1771‑ac, Notch 1 (rat): K1771‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEACETYLASE SIRT1 (human) activation of upstream enzyme, mutation in upstream enzyme recognition motif, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation modification-specific antibody
Downstream Regulation
Effect of modification (function):  protein stabilization, ubiquitination

K1772-ac - Notch 1 (mouse)
Modsite: VSEASkkkRREPLGE SwissProt Entrez-Gene
Orthologous residues
Notch 1 (human): K1782‑ac, Notch 1 (mouse): K1772‑ac, Notch 1 (rat): K1772‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEACETYLASE SIRT1 (human) activation of upstream enzyme, mutation in upstream enzyme recognition motif, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation modification-specific antibody
Downstream Regulation
Effect of modification (function):  protein stabilization, ubiquitination

K1785-ac - Notch 1 (mouse)
Modsite: GEDSVGLkPLKNAsD SwissProt Entrez-Gene
Orthologous residues
Notch 1 (human): K1795‑ac, Notch 1 (mouse): K1785‑ac, Notch 1 (rat): K1785‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEACETYLASE SIRT1 (human) activation of upstream enzyme, mutation in upstream enzyme recognition motif, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation modification-specific antibody
Downstream Regulation
Effect of modification (function):  protein stabilization, ubiquitination

K1935-ac - Notch 1 (mouse)
Modsite: YSRSDAAkRLLEASA SwissProt Entrez-Gene
Orthologous residues
Notch 1 (human): K1945‑ac, Notch 1 (mouse): K1935‑ac, Notch 1 (rat): K1935‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEACETYLASE SIRT1 (human) activation of upstream enzyme, mutation in upstream enzyme recognition motif, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation modification-specific antibody
Downstream Regulation
Effect of modification (function):  protein stabilization, ubiquitination

K2050-ac - Notch 1 (mouse)
Modsite: NKDMQNNkEETPLFL SwissProt Entrez-Gene
Orthologous residues
Notch 1 (human): R2060‑ac, Notch 1 (mouse): K2050‑ac, Notch 1 (rat): K2050‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEACETYLASE SIRT1 (human) activation of upstream enzyme, mutation in upstream enzyme recognition motif, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation modification-specific antibody
Downstream Regulation
Effect of modification (function):  protein stabilization, ubiquitination

K2068-ac - Notch 1 (mouse)
Modsite: EGSYETAkVLLDHFA SwissProt Entrez-Gene
Orthologous residues
Notch 1 (human): K2078‑ac, Notch 1 (mouse): K2068‑ac, Notch 1 (rat): K2068‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEACETYLASE SIRT1 (human) activation of upstream enzyme, mutation in upstream enzyme recognition motif, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation modification-specific antibody
Downstream Regulation
Effect of modification (function):  protein stabilization, ubiquitination

K2146-ac - Notch 1 (mouse)
Modsite: LKSATQGkkARkPsT SwissProt Entrez-Gene
Orthologous residues
Notch 1 (human): K2156‑ac, Notch 1 (mouse): K2146‑ac, Notch 1 (rat): K2146‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEACETYLASE SIRT1 (human) activation of upstream enzyme, mutation in upstream enzyme recognition motif, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation modification-specific antibody
Downstream Regulation
Effect of modification (function):  protein stabilization, ubiquitination

K2147-ac - Notch 1 (mouse)
Modsite: KSATQGkkARkPsTk SwissProt Entrez-Gene
Orthologous residues
Notch 1 (human): K2157‑ac, Notch 1 (mouse): K2147‑ac, Notch 1 (rat): K2147‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEACETYLASE SIRT1 (human) activation of upstream enzyme, mutation in upstream enzyme recognition motif, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation modification-specific antibody
Downstream Regulation
Effect of modification (function):  protein stabilization, ubiquitination

K2150-ac - Notch 1 (mouse)
Modsite: TQGkkARkPsTkGLA SwissProt Entrez-Gene
Orthologous residues
Notch 1 (human): K2160‑ac, Notch 1 (mouse): K2150‑ac, Notch 1 (rat): K2150‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEACETYLASE SIRT1 (human) activation of upstream enzyme, mutation in upstream enzyme recognition motif, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation modification-specific antibody
Downstream Regulation
Effect of modification (function):  protein stabilization, ubiquitination

K2154-ac - Notch 1 (mouse)
Modsite: kARkPsTkGLACGSk SwissProt Entrez-Gene
Orthologous residues
Notch 1 (human): K2164‑ac, Notch 1 (mouse): K2154‑ac, Notch 1 (rat): K2154‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEACETYLASE SIRT1 (human) activation of upstream enzyme, mutation in upstream enzyme recognition motif, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation modification-specific antibody
Downstream Regulation
Effect of modification (function):  protein stabilization, ubiquitination

K2161-ac - Notch 1 (mouse)
Modsite: kGLACGSkEAkDLKA SwissProt Entrez-Gene
Orthologous residues
Notch 1 (human): K2171‑ac, Notch 1 (mouse): K2161‑ac, Notch 1 (rat): K2161‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEACETYLASE SIRT1 (human) activation of upstream enzyme, mutation in upstream enzyme recognition motif, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation modification-specific antibody
Downstream Regulation
Effect of modification (function):  protein stabilization, ubiquitination

K2164-ac - Notch 1 (mouse)
Modsite: ACGSkEAkDLKARRK SwissProt Entrez-Gene
Orthologous residues
Notch 1 (human): K2174‑ac, Notch 1 (mouse): K2164‑ac, Notch 1 (rat): K2164‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEACETYLASE SIRT1 (human) activation of upstream enzyme, mutation in upstream enzyme recognition motif, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation modification-specific antibody
Downstream Regulation
Effect of modification (function):  protein stabilization, ubiquitination