Tey PY, et al. (2024) Rapid turnover of CTLA4 is associated with a complex architecture of reversible ubiquitylation. bioRxiv 38260548

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K203-ub - CTLA-4 (human) ▲

Modsite: LTTGVyVkMPPTEPE SwissProt Entrez-Gene Orthologous residues CTLA‑4 (human): K203‑ub, CTLA‑4 (mouse): K203‑ub, CTLA‑4 (rat): K203‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, western blotting Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  A2058 (keratinocyte), HeLa S3 Flp-In, T lymphocyte-spleen Cellular systems studied:  primary cells Species studied:  human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes DEUBIQUITINASE USP8 (human) transfection of inactive enzyme, transfection of wild-type enzyme, co-immunoprecipitation, siRNA inhibition of enzyme Comments:  K63, K27, and K29 linkages mixture may contribute to the rapidity of protein turnover Downstream Regulation Effect of modification (function):  enzymatic activity, induced, protein degradation Comments:  Catalytic activity and endosomal localization of USP8 are necessary for CTLA-4 deubiquitylation of K63,K27, and K29 linked attached chains for degradation, and rapid turnover of CTLA-4

K213-ub - CTLA-4 (human) ▲

Modsite: PTEPECEkQFQPyFI SwissProt Entrez-Gene Orthologous residues CTLA‑4 (human): K213‑ub, CTLA‑4 (mouse): K213‑ub, CTLA‑4 (rat): K213‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, western blotting Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  A2058 (keratinocyte), HeLa S3 Flp-In, T lymphocyte-spleen Cellular systems studied:  primary cells Species studied:  human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes DEUBIQUITINASE USP8 (human) transfection of inactive enzyme, transfection of wild-type enzyme, co-immunoprecipitation, siRNA inhibition of enzyme Comments:  K63, K27, and K29 linkages mixture may contribute to the rapidity of protein turnover Downstream Regulation Effect of modification (function):  enzymatic activity, induced, protein degradation Comments:  Catalytic activity and endosomal localization of USP8 are necessary for CTLA-4 deubiquitylation of K63,K27, and K29 linked attached chains for degradation, and rapid turnover of CTLA-4