Han Y, Bagchi P, Yun CC (2024) Regulation of the intestinal Na/H exchanger NHE3 by AMP-activated kinase is dependent on phosphorylation of NHE3 at S555 and S563. Am J Physiol Cell Physiol 326, C50-C59 38047302

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S555-p - NHE3 (human) ▲

Modsite: AEGERRGsLAFIRSP SwissProt Entrez-Gene Orthologous residues NHE3 (human): S555‑p, NHE3 (mouse): S550‑p, NHE3 (rat): S552‑p, NHE3 (rabbit): S554‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  C2BBe1 (intestinal) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE AMPKA1 (human) pharmacological activator of upstream enzyme, siRNA inhibition of enzyme KINASE AMPKA2 (human) pharmacological activator of upstream enzyme, siRNA inhibition of enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes acadesine increase siRNA decrease shAMPKA1 siRNA decrease shAMPKA2 siRNA acadesine inhibit treatment-induced increase shAMPKA1 siRNA acadesine inhibit treatment-induced increase shAMPKA2 NKH_477 increase H-89 NKH_477 inhibit treatment-induced increase H-89 acadesine no effect upon treatment-induced increase Downstream Regulation Effect of modification (function):  activity, inhibited, ubiquitination

S563-p - NHE3 (human) ▲

Modsite: LAFIRSPstDNVVNV SwissProt Entrez-Gene Orthologous residues NHE3 (human): S563‑p, NHE3 (mouse): S558‑p, NHE3 (rat): S560‑p, NHE3 (rabbit): S562‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site Disease tissue studied:  colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  C2BBe1 (intestinal) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE AMPKA1 (human) pharmacological activator of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes acadesine increase Downstream Regulation Effect of modification (function):  activity, inhibited, ubiquitination