Zhou M, Han Y, Jiang J (2023) Ulk4 promotes Shh signaling by regulating Stk36 ciliary localization and Gli2 phosphorylation. Elife 12 38096226

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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T1021-p - ULK4 (human) ▲

Modsite: AMTEHNPtFtRLVEE SwissProt Entrez-Gene Orthologous residues ULK4 (human): T1021‑p, ULK4 (mouse): A1021‑p, ULK4 (rat): A1021‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE Fused (mouse)

T1023-p - ULK4 (human) ▲

Modsite: TEHNPtFtRLVEESK SwissProt Entrez-Gene Orthologous residues ULK4 (human): T1023‑p, ULK4 (mouse): T1023‑p, ULK4 (rat): T1023‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE Fused (mouse) Downstream Regulation Effect of modification (function):  intracellular localization Effect of modification (process):  signaling pathway regulation Comments:  regulates the Hh pathway

T158-p - Fused (mouse) ▲

Modsite: STNTMVLtsIKGTPL SwissProt Entrez-Gene Orthologous residues Fused (human): T158‑p, Fused (mouse): T158‑p, Fused iso2 (mouse): T158‑p, Fused (rat): T158‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Fused (mouse) transfection of inactive enzyme, transfection of wild-type enzyme autophosphorylation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes SHH (mouse) increase ULK4 (mouse) SHH (mouse) no effect upon treatment-induced increase ULK4 KD has no effect

S159-p - Fused (mouse) ▲

Modsite: TNTMVLtsIKGTPLY SwissProt Entrez-Gene Orthologous residues Fused (human): S159‑p, Fused (mouse): S159‑p, Fused iso2 (mouse): S159‑p, Fused (rat): S159‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  3T3 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Fused (mouse) transfection of inactive enzyme, transfection of wild-type enzyme autophosphorylation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes SHH (mouse) increase ULK4 (mouse) SHH (mouse) no effect upon treatment-induced increase ULK4 KD has no effect

S230-p - GLI2 (mouse) ▲

Modsite: LSrKrALsIsPLSDA SwissProt Entrez-Gene Orthologous residues GLI2 (human): S234‑p, GLI2 iso1 (human): ‑, GLI2 iso2 (human): ‑, GLI2 (mouse): S230‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  3T3 (fibroblast), HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE Fused (mouse) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Fused (mouse) microscopy-colocalization, transfection of wild-type enzyme, co-immunoprecipitation, siRNA inhibition of enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes SHH (mouse) increase ULK4 (mouse) SHH (mouse) augment treatment-induced increase ULK4 KD decreases siRNA ULK4 (mouse) SHH (mouse) inhibit treatment-induced increase ULK4 and FUSED KD siRNA SHH (mouse) inhibit treatment-induced increase FUSED siRNA ULK3 (mouse) SHH (mouse) augment treatment-induced increase ULK3 KD inhibits ULK4 (mouse), ULK3 (mouse) SHH (mouse) augment treatment-induced increase ULK3 and ULK4 KD inhibits Downstream Regulation Effect of modification (process):  signaling pathway regulation Comments:  regulates the Hh pathway

S232-p - GLI2 (mouse) ▲

Modsite: rKrALsIsPLSDASL SwissProt Entrez-Gene Orthologous residues GLI2 (human): S236‑p, GLI2 iso1 (human): ‑, GLI2 iso2 (human): ‑, GLI2 (mouse): S232‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  3T3 (fibroblast), HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Fused (mouse) microscopy-colocalization, transfection of wild-type enzyme, co-immunoprecipitation, siRNA inhibition of enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes SHH (mouse) increase ULK4 (mouse) SHH (mouse) augment treatment-induced increase ULK4 KD decreases siRNA ULK4 (mouse) SHH (mouse) inhibit treatment-induced increase ULK4 and FUSED KD siRNA SHH (mouse) inhibit treatment-induced increase FUSED siRNA ULK3 (mouse) SHH (mouse) augment treatment-induced increase ULK3 KD inhibits ULK4 (mouse), ULK3 (mouse) SHH (mouse) augment treatment-induced increase ULK3 and ULK4 KD inhibits Downstream Regulation Effect of modification (process):  signaling pathway regulation Comments:  regulates the Hh pathway