Malik HS, et al. (2023) Phosphoprotein phosphatase activity positively regulates oligomeric pyrin to trigger inflammasome assembly in phagocytes. mBio, e0206623 37787552

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S208-p - pyrin (human) ▲
Modsite: VRLRRNAssAGRLQG SwissProt Entrez-Gene Orthologous residues pyrin (human): S208‑p, pyrin (mouse): S205‑p, pyrin (rat): Q204‑p

S242-p - pyrin (human) ▲

Modsite: SGKMRPRsLEVTIST SwissProt Entrez-Gene Orthologous residues pyrin (human): S242‑p, pyrin (mouse): S241‑p, pyrin (rat): V238‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  leukemia, acute myelogenous leukemia Relevant cell lines - cell types - tissues:  THP1 (myeloid) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes bacterial infection decrease B. cenocepacia calyculin_A bacterial infection inhibit treatment-induced decrease calyculin_A increase bacterial infection decrease Yersinia infection calyculin_A bacterial infection inhibit treatment-induced decrease tautomycetin bacterial infection no effect upon treatment-induced decrease

S205-p - pyrin (mouse) ▲

Modsite: AQLRRNVsSAGRLQG SwissProt Entrez-Gene Orthologous residues pyrin (human): S208‑p, pyrin (mouse): S205‑p, pyrin (rat): Q204‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  macrophage-bone marrow Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes PHOSPHATASE PPP2CB (human) siRNA inhibition of enzyme PHOSPHATASE PPP2CA (human) siRNA inhibition of enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes Clostridioides difficile toxin B decrease calyculin_A Clostridioides difficile toxin B inhibit treatment-induced decrease okadaic_acid Clostridioides difficile toxin B inhibit treatment-induced decrease ciclosporin Clostridioides difficile toxin B no effect upon treatment-induced decrease bacterial infection decrease Yersinia calyculin_A bacterial infection inhibit treatment-induced decrease siRNA bacterial infection inhibit treatment-induced decrease siPPP2CA Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays pyrin (human) Induces electrophoretic visualization

S241-p - pyrin (mouse) ▲
Modsite: SGKKRPRsLEITTYS SwissProt Entrez-Gene Orthologous residues pyrin (human): S242‑p, pyrin (mouse): S241‑p, pyrin (rat): V238‑p