Qiao X, et al. (2023) FBXO28 suppresses liver cancer invasion and metastasis by promoting PKA-dependent SNAI2 degradation. Oncogene 42, 2878-2891 37596321

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S158-p - Snail2 (human) ▲

Modsite: CDAQSRKsFSCKYCD SwissProt Entrez-Gene Orthologous residues Snail2 (human): S158‑p, Snail2 (mouse): S159‑p, Snail2 (rat): S158‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  liver cancer, hepatocellular carcinoma Relevant cell lines - cell types - tissues:  HCCLM3 (hepatic), Huh7 (hepatic) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKACA (human) pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes H-89 decrease KT5720 decrease Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation, ubiquitination Effect of modification (process):  carcinogenesis, inhibited, cell motility, inhibited Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays FBXO28 (human) Induces microscopy-colocalization, co-immunoprecipitation

S247-p - Snail2 (human) ▲

Modsite: KYQCKNCsKTFsRMs SwissProt Entrez-Gene Orthologous residues Snail2 (human): S247‑p, Snail2 (mouse): S248‑p, Snail2 (rat): S247‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  liver cancer, hepatocellular carcinoma Relevant cell lines - cell types - tissues:  HCCLM3 (hepatic), Huh7 (hepatic) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKACA (human) pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes H-89 decrease KT5720 decrease Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation, ubiquitination Effect of modification (process):  carcinogenesis, inhibited, cell motility, inhibited Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays FBXO28 (human) Induces carcinogenesis, inhibited, cell motility, inhibited microscopy-colocalization, co-immunoprecipitation

S254-p - Snail2 (human) ▲

Modsite: sKTFsRMsLLHKHEE SwissProt Entrez-Gene Orthologous residues Snail2 (human): S254‑p, Snail2 (mouse): S255‑p, Snail2 (rat): S254‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  liver cancer, hepatocellular carcinoma Relevant cell lines - cell types - tissues:  HCCLM3 (hepatic), Huh7 (hepatic) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKACA (human) pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes H-89 decrease KT5720 decrease Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation, ubiquitination Effect of modification (process):  carcinogenesis, inhibited, cell motility, inhibited Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays FBXO28 (human) Induces microscopy-colocalization, co-immunoprecipitation