Ghobashi AH, et al. (2023) Activation of AKT induces EZH2-mediated β-catenin trimethylation in colorectal cancer. iScience 26, 107630 37670785

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S473-p - Akt1 (human) ▲

Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene Orthologous residues Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Disease tissue studied:  colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  Caco-2 (intestinal), HEK293T (epithelial), RKO (intestinal) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes H2O2 increase EZH2 (human) no change compared to control EZH2 KD has no effect on AKT pS473 GSK690693 H2O2 inhibit treatment-induced increase PTEN (human) decrease PTEN KD increases AKT1 pS473 H2O2 PTEN (human) inhibit treatment-induced increase PTEN KD induces effect of H2O2 Downstream Regulation Effect of modification (function):  activity, induced, molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays EZH2 (human) Induces phosphorylation co-immunoprecipitation

K49-me - CTNNB1 (human) ▲

Modsite: TAPsLsGkGNPEEED SwissProt Entrez-Gene Orthologous residues CTNNB1 (human): K49‑me, CTNNB1 (mouse): K49‑me, CTNNB1 (rat): K49‑me, CTNNB1 (rabbit): K49‑me Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site Disease tissue studied:  colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  SW480 (intestinal) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes H2O2 increase PTEN (human) decrease PTEN KD increases CTNNB1 me-K49 decrease GSK-503 inhibits CTNNB1 K49-me Downstream Regulation Effect of modification (function):  intracellular localization, methylation, molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays POLR2A (human) Induces co-immunoprecipitation HNF1A (human) Induces co-immunoprecipitation

S21-p - EZH2 (human) ▲

Modsite: CWRKRVksEyMRLRQ SwissProt Entrez-Gene Orthologous residues EZH2 (human): S21‑p, EZH2 iso2 (human): S21‑p, EZH2 (mouse): S21‑p, EZH2 (rat): S21‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  LoVo (intestinal), RKO (intestinal), SW480 (intestinal) Cellular systems studied:  cell lines, tissue Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Akt1 (human) phospho-antibody, co-immunoprecipitation, activation of upstream enzyme, modification site within consensus motif, pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes H2O2 increase GSK690693 H2O2 inhibit treatment-induced increase Downstream Regulation Effect of modification (function):  enzymatic activity, induced, molecular association, regulation, phosphorylation Effect of modification (process):  cell motility, inhibited, chromatin organization, altered Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays POLR2A (human) Induces co-immunoprecipitation CTNNB1 (human) Induces co-immunoprecipitation