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Home > Curated Information Page > PubMed Id: 21487392
Cao Y, et al. (2011) LKB1 regulates TCR-mediated PLCĪ³1 activation and thymocyte positive selection. EMBO J 30, 2083-93 21487392
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y36-p - LKB1 (mouse)
Modsite: IDstEVIyQPRRKRA SwissProt Entrez-Gene
Orthologous residues
LKB1 (human): Y36‑p, LKB1 iso2 (human): Y36‑p, LKB1 (mouse): Y36‑p, LKB1 (rat): Y36‑p, LKB1 (pig):
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
Disease tissue studied:  leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Jurkat (T lymphocyte), thymocyte
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Lck (human) co-immunoprecipitation, genetic knockout/knockin of upstream enzyme, transfection of constitutively active enzyme, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
antibody increase
LckI antibody inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Effect of modification (process):  cell differentiation, induced, signaling pathway regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PLCG1 (mouse) Induces co-immunoprecipitation
LAT (mouse) Induces microscopy-colocalization
Comments:  TCR signaling, recruitment of PLCG1 to the LAT signalosome

Y261-p - LKB1 (mouse)
Modsite: PFEGDNIyKLFENIG SwissProt Entrez-Gene
Orthologous residues
LKB1 (human): Y261‑p, LKB1 iso2 (human): Y261‑p, LKB1 (mouse): Y261‑p, LKB1 (rat): Y261‑p, LKB1 (pig): Y167‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Jurkat (T lymphocyte), thymocyte
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Lck (human) co-immunoprecipitation, genetic knockout/knockin of upstream enzyme, transfection of constitutively active enzyme, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
antibody increase
LckI antibody inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Effect of modification (process):  cell differentiation, induced, signaling pathway regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
LAT (mouse) Induces microscopy-colocalization
PLCG1 (mouse) Induces co-immunoprecipitation
Comments:  TCR signaling, recruitment of PLCG1 to the LAT signalosome

Y365-p - LKB1 (mouse)
Modsite: DIEDGIIytQDFTVP SwissProt Entrez-Gene
Orthologous residues
LKB1 (human): Y362‑p, LKB1 iso2 (human): Y362‑p, LKB1 (mouse): Y365‑p, LKB1 (rat): Y365‑p, LKB1 (pig): Y270‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Jurkat (T lymphocyte), thymocyte
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Lck (human) co-immunoprecipitation, genetic knockout/knockin of upstream enzyme, transfection of constitutively active enzyme, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
antibody increase
LckI antibody inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Effect of modification (process):  cell differentiation, induced, signaling pathway regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PLCG1 (mouse) Induces co-immunoprecipitation
LAT (mouse) Induces microscopy-colocalization
Comments:  TCR signaling, recruitment of PLCG1 to the LAT signalosome