Kim JE, et al. (2023) Distinct Roles of CK2- and AKT-Mediated NF-κB Phosphorylations in Clasmatodendrosis (Autophagic Astroglial Death) within the Hippocampus of Chronic Epilepsy Rats. Antioxidants (Basel) 12 37237886

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S473-p - Akt1 (rat) ▲

Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene Orthologous residues Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p Characterization Methods used to characterize site in vivo:  immunoassay, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  astrocyte Cellular systems studied:  primary cells Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes NAC decrease in clasmatodendritic astrocytes NAC no change compared to control in reactive astrocytes TMCB decrease in clasmatodendritic astrocytes TMCB no change compared to control in reactive astrocytes Downstream Regulation Effect of modification (process):  autophagy, induced

Y255-p - CK2A1 (rat) ▲

Modsite: VLGTEDLyDYIDKYN SwissProt Entrez-Gene Orthologous residues CK2A1 (human): Y255‑p, CK2A1 (mouse): Y255‑p, CK2A1 (rat): Y255‑p, CK2A1 (rabbit): Y255‑p, CK2A1 (cow): Y255‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  astrocyte Cellular systems studied:  primary cells Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes NAC decrease 3CAI no change compared to control Downstream Regulation Effect of modification (process):  autophagy, induced Associated Diseases Diseases Alterations Comments Status epilepticus decreased

S528-p - NFkB-p65 (rat) ▲

Modsite: SGLPNGLsGDEDFSs SwissProt Entrez-Gene Orthologous residues NFkB‑p65 (human): S529‑p, NFkB‑p65 (mouse): S527‑p, NFkB‑p65 (rat): S528‑p Characterization Methods used to characterize site in vivo:  immunoassay, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'brain, hippocampus', astrocyte Cellular systems studied:  primary cells, tissue Species studied:  rat Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE CK2A1 (human) pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes NAC decrease in clasmatodendritic astrocytes NAC no change compared to control in reactive astrocytes TMCB decrease in clasmatodendritic astrocytes TMCB no change compared to control in reactive astrocytes 3CAI no change compared to control Downstream Regulation Effect of modification (process):  autophagy, induced

S535-p - NFkB-p65 (rat) ▲

Modsite: sGDEDFSsIADMDFS SwissProt Entrez-Gene Orthologous residues NFkB‑p65 (human): S536‑p, NFkB‑p65 (mouse): S534‑p, NFkB‑p65 (rat): S535‑p Characterization Methods used to characterize site in vivo:  immunoassay, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  astrocyte Cellular systems studied:  primary cells Species studied:  rat Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Akt1 (human) pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes NAC decrease in clasmatodendritic astrocytes NAC no change compared to control in reactive astrocytes TMCB decrease in clasmatodendritic astrocytes TMCB no change compared to control in reactive astrocytes 3CAI decrease (in clasmatodendritic astrocytes) 3CAI no change compared to control (in reactive astrocytes) Downstream Regulation Effect of modification (process):  autophagy, induced