Chen DQ, et al. (2023) Poricoic acid A suppresses renal fibroblast activation and interstitial fibrosis in UUO rats via upregulating Sirt3 and promoting β-catenin K49 deacetylation. Acta Pharmacol Sin 44, 1038-1050 36470978

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

Download

K19-ac - CTNNB1 (human) ▲

Modsite: MAMEPDRkAAVsHWQ SwissProt Entrez-Gene Orthologous residues CTNNB1 (human): K19‑ac, CTNNB1 (mouse): K19‑ac, CTNNB1 (rat): K19‑ac, CTNNB1 (rabbit): K19‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  NRK49F (fibroblast) Cellular systems studied:  cell lines Species studied:  human, rat

K49-ac - CTNNB1 (human) ▲

Modsite: TAPsLsGkGNPEEED SwissProt Entrez-Gene Orthologous residues CTNNB1 (human): K49‑ac, CTNNB1 (mouse): K49‑ac, CTNNB1 (rat): K49‑ac, CTNNB1 (rabbit): K49‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  NRK49F (fibroblast) Cellular systems studied:  cell lines Species studied:  human, rat

K19-ac - CTNNB1 (rat) ▲

Modsite: MAMEPDRkAAVSHWQ SwissProt Entrez-Gene Orthologous residues CTNNB1 (human): K19‑ac, CTNNB1 (mouse): K19‑ac, CTNNB1 (rat): K19‑ac, CTNNB1 (rabbit): K19‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  NRK49F (fibroblast) Cellular systems studied:  cell lines Species studied:  human, rat

K49-ac - CTNNB1 (rat) ▲

Modsite: TAPsLSGkGNPEEED SwissProt Entrez-Gene Orthologous residues CTNNB1 (human): K49‑ac, CTNNB1 (mouse): K49‑ac, CTNNB1 (rat): K49‑ac, CTNNB1 (rabbit): K49‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  NRK49F (fibroblast) Cellular systems studied:  cell lines Species studied:  human, rat Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes DEACETYLASE SIRT3 (rat) pharmacological activator of upstream enzyme, genetic knockout/knockin of upstream enzyme, transfection of wild-type enzyme, siRNA inhibition of enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PAA decrease Downstream Regulation Effect of modification (function):  activity, inhibited, protein degradation Effect of modification (process):  transcription, induced Comments:  Sirt3 mainly mediated ß-catenin K49 deacetylation and induced ubiquitin-dependent degradation, supressing pro¿brotic downstream target gene expression Associated Diseases Diseases Alterations Comments interstitial fibrosis increased