Ma X, et al. (2022) The E3 ubiquitin ligase MG53 inhibits hepatocellular carcinoma by targeting RAC1 signaling. Oncogenesis 11, 40 35858925

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K5-ub - RAC1 (human) ▲

Modsite: ___MQAIkCVVVGDG SwissProt Entrez-Gene Orthologous residues RAC1 (human): K5‑ub, RAC1 iso2 (human): K5‑ub, RAC1 (mouse): K5‑ub, RAC1 (rat): K5‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Disease tissue studied:  liver cancer, hepatocellular carcinoma Relevant cell lines - cell types - tissues:  Hep3B (hepatic), HepG2 (hepatic), Huh7 (hepatic), SNU-387 (hepatic) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE TRIM72 (human) siRNA inhibition of enzyme, co-immunoprecipitation, transfection of wild-type enzyme Downstream Regulation Effect of modification (process):  carcinogenesis, inhibited, signaling pathway regulation Comments:  RAC1-MAPK signaling axis, Trim72 signi¿cantly inhibited the tumorigenesis of HCC i

K48-ub - ubiquitin (human) ▲

Modsite: QRLIFAGkQLEDGRT SwissProt Orthologous residues ubiquitin (human): K48‑ub, ubiquitin (mouse): K48‑ub, ubiquitin (rat): K48‑ub, ubiquitin (cow): K48‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Disease tissue studied:  liver cancer, hepatocellular carcinoma Relevant cell lines - cell types - tissues:  Hep3B (hepatic), HepG2 (hepatic), Huh7 (hepatic), SNU-387 (hepatic) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE TRIM72 (human) siRNA inhibition of enzyme, co-immunoprecipitation, transfection of wild-type enzyme Downstream Regulation Effect of modification (process):  carcinogenesis, inhibited, signaling pathway regulation Comments:  RAC1-MAPK signaling axis, Trim72 signi¿cantly inhibited the tumorigenesis of HCC i