Liu YY, et al. (2021) Thyroid hormone receptor alpha sumoylation modulates white adipose tissue stores. Sci Rep 11, 24105 34916557

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K283-sm - TR-alpha (mouse) ▲

Modsite: LSGEMAVkREQLkNG SwissProt Entrez-Gene Orthologous residues TR‑alpha (human): K283‑sm, TR‑alpha iso2 (human): K283‑sm, TR‑alpha (mouse): K283‑sm, TR‑alpha iso2 (mouse): K283‑sm, TR‑alpha iso3 (mouse): K283‑sm, TR‑alpha iso5 (mouse): K283‑sm, TR‑alpha (rat): K283‑sm, TR‑alpha iso2 (rat): K283‑sm, TR‑alpha (chicken): K281‑sm Characterization Methods used to characterize site in vivo:  flow cytometry, immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  preadipocyte Cellular systems studied:  cell lines, primary cells Species studied:  human, mouse Downstream Regulation Effect of modification (function):  intracellular localization Effect of modification (process):  cell cycle regulation, cell growth, induced, transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces co-immunoprecipitation Comments:  (K283Q/K288R)-/- mutant: reduced nuclear localization of cyclin D1, cyclin D2, Cdk4 and Cdk2 in human primary preadipocytes; decreased preadipocyte proliferation, reduced white adipose stores and adipocyte cell diameter in mutant mice; reduced CREB and AP1-mediated gene expression, reduced TR-alpha–CREB interaction in mutant preadipocytes; mutant mice had reduced fat stores on normal chow, and was reduced, more on high fat diets

K288-sm - TR-alpha (mouse) ▲

Modsite: AVkREQLkNGGLGVV SwissProt Entrez-Gene Orthologous residues TR‑alpha (human): K288‑sm, TR‑alpha iso2 (human): K288‑sm, TR‑alpha (mouse): K288‑sm, TR‑alpha iso2 (mouse): K288‑sm, TR‑alpha iso3 (mouse): K288‑sm, TR‑alpha iso5 (mouse): K288‑sm, TR‑alpha (rat): K288‑sm, TR‑alpha iso2 (rat): K288‑sm, TR‑alpha (chicken): K286‑sm Characterization Methods used to characterize site in vivo:  flow cytometry, immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  preadipocyte Cellular systems studied:  cell lines, primary cells Species studied:  human, mouse Downstream Regulation Effect of modification (function):  intracellular localization Effect of modification (process):  cell cycle regulation, cell growth, induced, transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces co-immunoprecipitation Comments:  (K283Q/K288R)-/- mutant: reduced nuclear localization of cyclin D1, cyclin D2, Cdk4 and Cdk2 in human primary preadipocytes; decreased preadipocyte proliferation, reduced white adipose stores and adipocyte cell diameter in mutant mice; reduced CREB and AP1-mediated gene expression, reduced TR-alpha–CREB interaction in mutant preadipocytes; mutant mice had reduced fat stores on normal chow, and was reduced, more on high fat diets