Ueda S, et al. (2022) Polo-Like Kinase 2 Plays an Essential Role in Cytoprotection against MG132-Induced Proteasome Inhibition via Phosphorylation of Serine 19 in HSPB5. Int J Mol Sci 23 36232565

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S19-p - CRYAB (human) ▲

Modsite: RPFFPFHsPsrLFDQ SwissProt Entrez-Gene Orthologous residues CRYAB (human): S19‑p, CRYAB (mouse): S19‑p, CRYAB (rat): S19‑p, CRYAB (rabbit): S19‑p, CRYAB (pig): S19‑p, CRYAB (cow): S19‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), L6 (myoblast) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PLK2 (human) siRNA inhibition of enzyme, microscopy-colocalization, pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes MG132 increase BI2536 MG132 inhibit treatment-induced increase siRNA decrease PLK2 siRNA Downstream Regulation Effect of modification (process):  apoptosis, inhibited

S59-p - CRYAB (human) ▲

Modsite: PsFLRAPsWFDTGLS SwissProt Entrez-Gene Orthologous residues CRYAB (human): S59‑p, CRYAB (mouse): S59‑p, CRYAB (rat): S59‑p, CRYAB (rabbit): S59‑p, CRYAB (pig): S59‑p, CRYAB (cow): S59‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), L6 (myoblast) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes MG132 increase MG132 PLK2 (human) augment treatment-induced increase PLK2 KD inhibits Downstream Regulation Effect of modification (process):  apoptosis, inhibited