Chen B, et al. (2022) Gut bacteria alleviate smoking-related NASH by degrading gut nicotine. Nature 610, 562-568 36261549

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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T183-p - AMPKA1 (human) ▲

Modsite: sDGEFLRtsCGsPNy SwissProt Entrez-Gene Orthologous residues AMPKA1 (human): T183‑p, AMPKA1 iso2 (human): T198‑p, AMPKA1 (mouse): T183‑p, AMPKA1 (rat): T183‑p, AMPKA1 (fruit fly): T184‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  colorectal cancer, colorectal carcinoma, NAFLD Relevant cell lines - cell types - tissues:  SW480 (intestinal) Cellular systems studied:  cell lines Species studied:  bacteria, human, mouse Comments:  ileum mucosa biopsy, serum and stool samples were collected from 30 non-smokers and 30 smokers Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes cigarette_smoke increase low nicotine cigarette_smoke cigarette_smoke augment treatment-induced increase high nicotine

K103-ub - SMPD3 (human) ▲

Modsite: IYSRLEDkGLAGGAA SwissProt Entrez-Gene Orthologous residues SMPD3 (human): K103‑ub, SMPD3 (mouse): K103‑ub, SMPD3 (rat): K103‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  colorectal cancer, colorectal carcinoma, NAFLD Relevant cell lines - cell types - tissues:  SW480 (intestinal) Cellular systems studied:  cell lines Species studied:  bacteria, human, mouse Comments:  ileum mucosa biopsy, serum and stool samples were collected from 30 non-smokers and 30 smokers Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes cigarette_smoke decrease low nicotine cigarette_smoke augment treatment-induced decrease high nicotine Downstream Regulation Effect of modification (function):  protein degradation

S209-p - SMPD3 (human) ▲

Modsite: GsIKRTAsVEyKGDG SwissProt Entrez-Gene Orthologous residues SMPD3 (human): S209‑p, SMPD3 (mouse): S208‑p, SMPD3 (rat): S208‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  colorectal cancer, colorectal carcinoma, NAFLD Relevant cell lines - cell types - tissues:  SW480 (intestinal) Cellular systems studied:  cell lines Species studied:  bacteria, human, mouse Comments:  ileum mucosa biopsy, serum and stool samples were collected from 30 non-smokers and 30 smokers Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE AMPKA1 (human) co-immunoprecipitation, pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes cigarette_smoke increase low nicotine cigarette_smoke cigarette_smoke augment treatment-induced increase high nicotine Downstream Regulation Effect of modification (function):  protein stabilization Comments:  phosphorylation of SMPD3 S208 inhibits degradation of K103-mediated degradation of SMPD3

K48-ub - ubiquitin (human) ▲

Modsite: QRLIFAGkQLEDGRT SwissProt Orthologous residues ubiquitin (human): K48‑ub, ubiquitin (mouse): K48‑ub, ubiquitin (rat): K48‑ub, ubiquitin (cow): K48‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  colorectal cancer, colorectal carcinoma, NAFLD Relevant cell lines - cell types - tissues:  SW480 (intestinal) Cellular systems studied:  cell lines Species studied:  bacteria, human, mouse Comments:  ileum mucosa biopsy, serum and stool samples were collected from 30 non-smokers and 30 smokers Downstream Regulation Effect of modification (function):  protein degradation

T183-p - AMPKA1 (mouse) ▲

Modsite: sDGEFLRtsCGsPNY SwissProt Entrez-Gene Orthologous residues AMPKA1 (human): T183‑p, AMPKA1 iso2 (human): T198‑p, AMPKA1 (mouse): T183‑p, AMPKA1 (rat): T183‑p, AMPKA1 (fruit fly): T184‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  colorectal cancer, colorectal carcinoma, NAFLD Relevant cell lines - cell types - tissues:  SW480 (intestinal) Cellular systems studied:  cell lines Species studied:  bacteria, human, mouse Comments:  ileum mucosa biopsy, serum and stool samples were collected from 30 non-smokers and 30 smokers

K103-ub - SMPD3 (mouse) ▲

Modsite: SYSRLEDkNPAGGAA SwissProt Entrez-Gene Orthologous residues SMPD3 (human): K103‑ub, SMPD3 (mouse): K103‑ub, SMPD3 (rat): K103‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  colorectal cancer, colorectal carcinoma, NAFLD Relevant cell lines - cell types - tissues:  SW480 (intestinal) Cellular systems studied:  cell lines Species studied:  bacteria, human, mouse Comments:  ileum mucosa biopsy, serum and stool samples were collected from 30 non-smokers and 30 smokers Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes nicotine decrease nicotine water B. xylanisolvens colonization nicotine inhibit treatment-induced decrease Downstream Regulation Effect of modification (function):  protein degradation

S208-p - SMPD3 (mouse) ▲

Modsite: GsIKRTAsVEYKGDG SwissProt Entrez-Gene Orthologous residues SMPD3 (human): S209‑p, SMPD3 (mouse): S208‑p, SMPD3 (rat): S208‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  colorectal cancer, colorectal carcinoma, NAFLD Relevant cell lines - cell types - tissues:  SW480 (intestinal) Cellular systems studied:  cell lines Species studied:  bacteria, human, mouse Comments:  ileum mucosa biopsy, serum and stool samples were collected from 30 non-smokers and 30 smokers Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE AMPKA1 (human) co-immunoprecipitation, pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes nicotine increase nicotine water B. xylanisolvens colonization nicotine inhibit treatment-induced increase Downstream Regulation Effect of modification (function):  protein stabilization Comments:  phosphorylation of SMPD3 S208 inhibits degradation of K103-mediated degradation of SMPD3

K48-ub - ubiquitin (mouse) ▲

Modsite: QRLIFAGkQLEDGRT SwissProt Orthologous residues ubiquitin (human): K48‑ub, ubiquitin (mouse): K48‑ub, ubiquitin (rat): K48‑ub, ubiquitin (cow): K48‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  colorectal cancer, colorectal carcinoma, NAFLD Relevant cell lines - cell types - tissues:  SW480 (intestinal) Cellular systems studied:  cell lines Species studied:  bacteria, human, mouse Comments:  ileum mucosa biopsy, serum and stool samples were collected from 30 non-smokers and 30 smokers Downstream Regulation Effect of modification (function):  protein degradation