Zhang Y, et al. (2019) USP16-mediated deubiquitination of calcineurin A controls peripheral T cell maintenance. J Clin Invest 129, 2856-2871 31135381

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K327-ub - PPP3CB (human) ▲

Modsite: YLDVYNNkAAVLkYE SwissProt Entrez-Gene Orthologous residues PPP3CB (human): K327‑ub, PPP3CB iso4 (human): K327‑ub, PPP3CB (mouse): K327‑ub, PPP3CB (rat): K327‑ub Characterization Methods used to characterize site in vivo:  flow cytometry, immunoprecipitation, mass spectrometry, western blotting Disease tissue studied:  lymphoma, T cell lymphoma Relevant cell lines - cell types - tissues:  EL-4 (T lymphocyte), HEK293T (epithelial), lymphocyte Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes DEUBIQUITINASE USP16 (human) transfection of wild-type enzyme, transfection of inactive enzyme Downstream Regulation Effect of modification (process):  cell cycle regulation, cell growth, inhibited, transcription, inhibited Comments:  Ubiquitination of PPP3CB impairs NFAT recruitment and transcription of NFAT-targeted genes; USP16 regulates T cell proliferation and cell-cycle entry

K29-ub - ubiquitin (human) ▲

Modsite: tIENVkAkIQDkEGI SwissProt Orthologous residues ubiquitin (human): K29‑ub, ubiquitin (mouse): K29‑ub, ubiquitin (rat): K29‑ub, ubiquitin (cow): K29‑ub Characterization Methods used to characterize site in vivo:  flow cytometry, immunoprecipitation, mass spectrometry, western blotting Disease tissue studied:  lymphoma, T cell lymphoma Relevant cell lines - cell types - tissues:  EL-4 (T lymphocyte), HEK293T (epithelial), lymphocyte Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes DEUBIQUITINASE USP16 (human) transfection of wild-type enzyme, transfection of inactive enzyme Downstream Regulation Effect of modification (process):  cell cycle regulation, cell growth, inhibited, transcription, inhibited Comments:  Ubiquitination of PPP3CB impairs NFAT recruitment and transcription of NFAT-targeted genes; USP16 regulates T cell proliferation and cell-cycle entry

K327-ub - PPP3CB (mouse) ▲

Modsite: YLDVYNNkAAVLkYE SwissProt Entrez-Gene Orthologous residues PPP3CB (human): K327‑ub, PPP3CB iso4 (human): K327‑ub, PPP3CB (mouse): K327‑ub, PPP3CB (rat): K327‑ub Characterization Methods used to characterize site in vivo:  flow cytometry, immunoprecipitation, mass spectrometry, western blotting Disease tissue studied:  lymphoma, T cell lymphoma Relevant cell lines - cell types - tissues:  EL-4 (T lymphocyte), HEK293T (epithelial), lymphocyte Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes DEUBIQUITINASE USP16 (human) transfection of wild-type enzyme, transfection of inactive enzyme Downstream Regulation Effect of modification (process):  cell cycle regulation, cell growth, inhibited, transcription, inhibited Comments:  Ubiquitination of PPP3CB impairs NFAT recruitment and transcription of NFAT-targeted genes; USP16 regulates T cell proliferation and cell-cycle entry

K29-ub - ubiquitin (mouse) ▲

Modsite: TIENVkAkIQDkEGI SwissProt Orthologous residues ubiquitin (human): K29‑ub, ubiquitin (mouse): K29‑ub, ubiquitin (rat): K29‑ub, ubiquitin (cow): K29‑ub Characterization Methods used to characterize site in vivo:  flow cytometry, immunoprecipitation, mass spectrometry, western blotting Disease tissue studied:  lymphoma, T cell lymphoma Relevant cell lines - cell types - tissues:  EL-4 (T lymphocyte), HEK293T (epithelial), lymphocyte Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes DEUBIQUITINASE USP16 (human) transfection of wild-type enzyme, transfection of inactive enzyme Downstream Regulation Effect of modification (process):  cell cycle regulation, cell growth, inhibited, transcription, inhibited Comments:  Ubiquitination of PPP3CB impairs NFAT recruitment and transcription of NFAT-targeted genes; USP16 regulates T cell proliferation and cell-cycle entry