Lee SB, et al. (2022) Regulated interaction of ID2 with the anaphase-promoting complex links progression through mitosis with reactivation of cell-type-specific transcription. Nat Commun 13, 2089 35440621

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S5-p - ID2 (human) ▲

Modsite: ___MKAFsPVRSVRk SwissProt Entrez-Gene Orthologous residues ID2 (human): S5‑p, ID2 (mouse): S5‑p, ID2 (rat): S5‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  brain cancer, glioblastoma, glioma Relevant cell lines - cell types - tissues:  HeLa (cervical), U-251MG (glioma) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE CDK1 (human) Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes dinaciclib decrease palbociclib no change compared to control Downstream Regulation Effect of modification (function):  molecular association, regulation, protein stabilization, ubiquitination Effect of modification (process):  cell cycle regulation, transcription, altered Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays APC (human) Disrupts pull-down assay, co-immunoprecipitation Comments:  inhibits ubiquitination

S14-p - ID2 (human) ▲
Modsite: VRSVRkNsLsDHSLG SwissProt Entrez-Gene Orthologous residues ID2 (human): S14‑p, ID2 (mouse): S14‑p, ID2 (rat): S14‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human

T27-p - ID2 (human) ▲
Modsite: LGISRSKtPVDDPMS SwissProt Entrez-Gene Orthologous residues ID2 (human): T27‑p, ID2 (mouse): T27‑p, ID2 (rat): T27‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human