Zhang A, et al. (2022) USP33 deubiquitinates and stabilizes HIF-2alpha to promote hypoxia response in glioma stem cells. EMBO J 41, e109187 35191554

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S484-p - HIF2A (human) ▲

Modsite: SSCSTPNsPEDYYTS SwissProt Entrez-Gene Orthologous residues HIF2A (human): S484‑p, HIF2A (mouse): S483‑p, HIF2A (rat): S483‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  HEK293T (epithelial), T387 (glioma) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE ERK1 (human) pharmacological inhibitor of upstream enzyme, phospho-antibody KINASE ERK2 (human) pharmacological inhibitor of upstream enzyme, phospho-antibody Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes LY3214996 decrease U0126 decrease Downstream Regulation Effect of modification (function):  molecular association, regulation, protein stabilization, ubiquitination Effect of modification (process):  apoptosis, inhibited, carcinogenesis, induced, cell growth, induced, transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays USP33 (human) Induces cell growth, induced, carcinogenesis, induced, transcription, induced, apoptosis, inhibited co-immunoprecipitation Comments:  induces deubiquitination

S581-p - HIF2A (human) ▲
Modsite: LAPVAPHsPFLLDKF SwissProt Entrez-Gene Orthologous residues HIF2A (human): S581‑p, HIF2A (mouse): G580‑p, HIF2A (rat): G580‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human