Miyamoto Y, et al. (2022) The adaptor SH2B1 and the phosphatase PTP4A1 regulate the phosphorylation of cytohesin-2 in myelinating Schwann cells in mice. Sci Signal 15, eabi5276 35077201

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

Download

Y382-p - cytohesin 1 (mouse) ▲
Modsite: AISRDPFyEMLAARK SwissProt Entrez-Gene Orthologous residues cytohesin 1 (human): Y382‑p, cytohesin 1 (mouse): Y382‑p, cytohesin 1 (rat): Y382‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  sciatic nerve Cellular systems studied:  tissue Species studied:  mouse

Y381-p - cytohesin 2 (mouse) ▲

Modsite: AVSVDPFyEMLAARK SwissProt Entrez-Gene Orthologous residues cytohesin 2 (human): Y381‑p, cytohesin 2 (mouse): Y381‑p, cytohesin 2 (rat): Y381‑p Characterization Methods used to characterize site in vivo:  immunoassay, immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), RT-4 Schwannoma (Schwann), sciatic nerve Cellular systems studied:  cell lines, tissue Species studied:  human, mouse, rat Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Fyn (mouse) transfection of wild-type enzyme PHOSPHATASE PTP4A1 (mouse) co-immunoprecipitation, siRNA inhibition of enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes siRNA increase PTP4A1 siRNA SH2-B-beta (human) increase SH2B KD decreases PTPN13 (human) no change compared to control PTP1B (human) no change compared to control PTPN2 (human) no change compared to control Downstream Regulation Effect of modification (function):  activity, induced, molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays GGA3 (human) Induces affinity chromatography SH2-B-beta (human) Induces co-immunoprecipitation, in vitro ARF6 (human) Induces affinity chromatography