Yang PB, et al. (2020) Blocking PPARγ interaction facilitates Nur77 interdiction of fatty acid uptake and suppresses breast cancer progression. Proc Natl Acad Sci U S A 117, 27412-27422 33087562

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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K9-ac - H3 (human) ▲

Modsite: rtkQtArkstGGkAP SwissProt Entrez-Gene Orthologous residues H3 (human): K9‑ac, H3 (mouse): K9‑ac, H3 iso2 (mouse): K9‑ac, H3 iso3 (mouse): K9‑ac, H3 (rat): K9‑ac, H3 iso3 (rat): K9‑ac, H3 (pig): K9‑ac, H3 (chicken): K9‑ac, H3 (cow): K9‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  breast, MCF-7 (breast cell) Cellular systems studied:  cell lines Species studied:  human, mouse Downstream Regulation Effect of modification (function):  protein degradation

K8-ac - H4 (human) ▲

Modsite: sGrGkGGkGLGkGGA SwissProt Entrez-Gene Orthologous residues H4 (human): K8‑ac, H4 (mouse): K8‑ac, H4 (rat): K8‑ac, H4 (pig): K8‑ac, H4 (cow): K8‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  breast, MCF-7 (breast cell) Cellular systems studied:  cell lines Species studied:  human, mouse Downstream Regulation Effect of modification (function):  protein degradation

K536-ub - Nur77 (human) ▲

Modsite: NRIASCLkEHVAAVA SwissProt Entrez-Gene Orthologous residues Nur77 (human): K536‑ub, Nur77 iso2 (human): K549‑ub, Nur77 (mouse): K539‑ub, Nur77 (rat): K535‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  breast, MCF-7 (breast cell) Cellular systems studied:  cell lines Species studied:  human, mouse Downstream Regulation Effect of modification (function):  protein degradation

K9-ac - H3 (mouse) ▲

Modsite: RtkQTARkstGGkAP SwissProt Entrez-Gene Orthologous residues H3 (human): K9‑ac, H3 (mouse): K9‑ac, H3 iso2 (mouse): K9‑ac, H3 iso3 (mouse): K9‑ac, H3 (rat): K9‑ac, H3 iso3 (rat): K9‑ac, H3 (pig): K9‑ac, H3 (chicken): K9‑ac, H3 (cow): K9‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  breast, MCF-7 (breast cell) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE TRIM13 (mouse) siRNA inhibition of enzyme, co-immunoprecipitation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes Nur77 (mouse) decrease Downstream Regulation Effect of modification (function):  protein degradation

K8-ac - H4 (mouse) ▲

Modsite: SGrGkGGkGLGkGGA SwissProt Entrez-Gene Orthologous residues H4 (human): K8‑ac, H4 (mouse): K8‑ac, H4 (rat): K8‑ac, H4 (pig): K8‑ac, H4 (cow): K8‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  breast, MCF-7 (breast cell) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE TRIM13 (mouse) siRNA inhibition of enzyme, co-immunoprecipitation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes Nur77 (mouse) decrease Downstream Regulation Effect of modification (function):  protein degradation

K539-ub - Nur77 (mouse) ▲

Modsite: NRIASCLkEHMATVA SwissProt Entrez-Gene Orthologous residues Nur77 (human): K536‑ub, Nur77 iso2 (human): K549‑ub, Nur77 (mouse): K539‑ub, Nur77 (rat): K535‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  breast, MCF-7 (breast cell) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE TRIM13 (mouse) siRNA inhibition of enzyme, co-immunoprecipitation Downstream Regulation Effect of modification (function):  protein degradation