Zhou Q, et al. (2021) Up-regulation of PUMA caused the activation of p53 phosphorylation and acetylation, enhancing the interaction between PUMA and Bcl-X and mediating arsenic-induced apoptosis. Toxicol Appl Pharmacol 434, 115800 34798143

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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T55-p - p53 (human) ▲

Modsite: DDIEQWFtEDPGPDE SwissProt Entrez-Gene Orthologous residues p53 (human): T55‑p, p53 iso2 (human): T55‑p, p53 iso4 (human): T16‑p, p53 (mouse): E55‑p, p53 iso2 (mouse): E55‑p, p53 (rat): E57‑p, p53 (rabbit): N54‑p, p53 (green monkey): T55‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma Relevant cell lines - cell types - tissues:  16HBE (epithelial), A549 (pulmonary) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes NaAsO2 increase Downstream Regulation Effect of modification (process):  apoptosis, induced Comments:  arsenic induced overexpression of PUMA, P53 phosphorylation and acetylation, activation and accumulation of p53, PUMA disruption of Bcl-X complexes with pro-survival proteins resulting in apoptosis

S315-p - p53 (human) ▲

Modsite: LPNNtsssPQPkkkP SwissProt Entrez-Gene Orthologous residues p53 (human): S315‑p, p53 iso2 (human): S315‑p, p53 iso4 (human): S276‑p, p53 (mouse): S312‑p, p53 iso2 (mouse): S312‑p, p53 (rat): S313‑p, p53 (rabbit): S313‑p, p53 (green monkey): S315‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma Relevant cell lines - cell types - tissues:  16HBE (epithelial), A549 (pulmonary) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes NaAsO2 increase Downstream Regulation Effect of modification (process):  apoptosis, induced Comments:  arsenic induced overexpression of PUMA, P53 phosphorylation and acetylation, activation and accumulation of p53, PUMA disruption of Bcl-X complexes with pro-survival proteins resulting in apoptosis

K370-ac - p53 (human) ▲

Modsite: RAHssHLkskkGQst SwissProt Entrez-Gene Orthologous residues p53 (human): K370‑ac, p53 iso2 (human): ‑, p53 iso4 (human): K331‑ac, p53 (mouse): K367‑ac, p53 iso2 (mouse): ‑, p53 (rat): K368‑ac, p53 (rabbit): K368‑ac, p53 (green monkey): K370‑ac Characterization Methods used to characterize site in vivo:  modification-specific antibody, western blotting Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma Relevant cell lines - cell types - tissues:  16HBE (epithelial), A549 (pulmonary) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes NaAsO2 increase Downstream Regulation Effect of modification (process):  apoptosis, induced Comments:  arsenic induced overexpression of PUMA, P53 phosphorylation and acetylation, activation and accumulation of p53, PUMA disruption of Bcl-X complexes with pro-survival proteins resulting in apoptosis

S376-p - p53 (human) ▲

Modsite: LkskkGQstsRHkkL SwissProt Entrez-Gene Orthologous residues p53 (human): S376‑p, p53 iso2 (human): ‑, p53 iso4 (human): S337‑p, p53 (mouse): S373‑p, p53 iso2 (mouse): ‑, p53 (rat): S374‑p, p53 (rabbit): S374‑p, p53 (green monkey): S376‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma Relevant cell lines - cell types - tissues:  16HBE (epithelial), A549 (pulmonary) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes NaAsO2 increase Downstream Regulation Effect of modification (process):  apoptosis, induced Comments:  arsenic induced overexpression of PUMA, P53 phosphorylation and acetylation, activation and accumulation of p53, PUMA disruption of Bcl-X complexes with pro-survival proteins resulting in apoptosis

K382-ac - p53 (human) ▲

Modsite: QstsRHkkLMFktEG SwissProt Entrez-Gene Orthologous residues p53 (human): K382‑ac, p53 iso2 (human): ‑, p53 iso4 (human): K343‑ac, p53 (mouse): K379‑ac, p53 iso2 (mouse): ‑, p53 (rat): K380‑ac, p53 (rabbit): K380‑ac, p53 (green monkey): K382‑ac Characterization Methods used to characterize site in vivo:  modification-specific antibody, western blotting Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma Relevant cell lines - cell types - tissues:  16HBE (epithelial), A549 (pulmonary) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes NaAsO2 increase Downstream Regulation Effect of modification (process):  apoptosis, induced Comments:  arsenic induced overexpression of PUMA, P53 phosphorylation and acetylation, activation and accumulation of p53, PUMA disruption of Bcl-X complexes with pro-survival proteins resulting in apoptosis

S392-p - p53 (human) ▲

Modsite: FktEGPDsD______ SwissProt Entrez-Gene Orthologous residues p53 (human): S392‑p, p53 iso2 (human): ‑, p53 iso4 (human): S353‑p, p53 (mouse): S389‑p, p53 iso2 (mouse): ‑, p53 (rat): S390‑p, p53 (rabbit): S390‑p, p53 (green monkey): S392‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma Relevant cell lines - cell types - tissues:  16HBE (epithelial), A549 (pulmonary) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes NaAsO2 increase Downstream Regulation Effect of modification (process):  apoptosis, induced Comments:  arsenic induced overexpression of PUMA, P53 phosphorylation and acetylation, activation and accumulation of p53, PUMA disruption of Bcl-X complexes with pro-survival proteins resulting in apoptosis