Wang K, et al. (2021) Phosphorylation at Ser68 facilitates DCAF11-mediated ubiquitination and degradation of CENP-A during the cell cycle. Cell Rep 37, 109987 34758320

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K49-ub - CENPA (human) ▲

Modsite: rRRQGWLkEIRKLQK SwissProt Entrez-Gene Orthologous residues CENPA (human): K49‑ub, CENPA (mouse): K43‑ub, CENPA (rat): K68‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE CUL4A (human) co-immunoprecipitation Downstream Regulation Effect of modification (function):  intracellular localization, protein degradation Effect of modification (process):  cell cycle regulation Comments:  Ser68 facilitates degradation

S68-p - CENPA (human) ▲

Modsite: LIRKLPFsRLAREIC SwissProt Entrez-Gene Orthologous residues CENPA (human): S68‑p, CENPA (mouse): S62‑p, CENPA (rat): G87‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (function):  intracellular localization, protein degradation Effect of modification (process):  cell cycle regulation Comments:  Ser68 facilitates degradation

K124-ub - CENPA (human) ▲

Modsite: GRVTLFPkDVQLARR SwissProt Entrez-Gene Orthologous residues CENPA (human): K124‑ub, CENPA (mouse): K118‑ub, CENPA (rat): K143‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE CUL4A (human) co-immunoprecipitation Downstream Regulation Effect of modification (function):  intracellular localization, protein degradation Effect of modification (process):  cell cycle regulation Comments:  Ser68 facilitates degradation