Yu JE, et al. (2021) Phosphorylation of β-catenin Ser60 by polo-like kinase 1 drives the completion of cytokinesis. EMBO Rep, e51503 34585824

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S60-p - CTNNB1 (human) ▲

Modsite: EEEDVDTsQVLYEWE SwissProt Entrez-Gene Orthologous residues CTNNB1 (human): S60‑p, CTNNB1 (mouse): S60‑p, CTNNB1 (rat): S60‑p, CTNNB1 (rabbit): S60‑p Characterization Methods used to characterize site in vivo:  immunoassay, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE PLK1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PLK1 (human) phospho-antibody, transfection of constitutively active enzyme, transfection of wild-type enzyme, microscopy-colocalization, transfection of inactive enzyme, siRNA inhibition of enzyme, co-immunoprecipitation, pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes BI2536 decrease siRNA decrease PLK1 shRNA Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  cell cycle regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays ECT2 (human) Induces intracellular localization microscopy-colocalization, co-immunoprecipitation

S60-p - CTNNB1 (mouse) ▲

Modsite: EEEDVDTsQVLYEWE SwissProt Entrez-Gene Orthologous residues CTNNB1 (human): S60‑p, CTNNB1 (mouse): S60‑p, CTNNB1 (rat): S60‑p, CTNNB1 (rabbit): S60‑p Characterization Methods used to characterize site in vivo:  immunoassay, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE PLK1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PLK1 (human) phospho-antibody, transfection of constitutively active enzyme, transfection of wild-type enzyme, microscopy-colocalization, transfection of inactive enzyme, siRNA inhibition of enzyme, co-immunoprecipitation, pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes BI2536 decrease siRNA decrease PLK1 shRNA Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  cell cycle regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays ECT2 (human) Induces intracellular localization microscopy-colocalization, co-immunoprecipitation