Wan X, et al. (2020) Cisplatin inhibits SIRT3-deacetylation MTHFD2 to disturb cellular redox balance in colorectal cancer cell. Cell Death Dis 11, 649 32811824

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K44-ac - MTHFD2 (human) ▲
Modsite: AVVISGRkLAQQIkQ SwissProt Entrez-Gene Orthologous residues MTHFD2 (human): K44‑ac, MTHFD2 (mouse): K44‑ac, MTHFD2 (rat): K43‑ac Characterization Methods used to characterize site in vivo:  mass spectrometry (in vitro)

K50-ac - MTHFD2 (human) ▲
Modsite: RkLAQQIkQEVRQEV SwissProt Entrez-Gene Orthologous residues MTHFD2 (human): K50‑ac, MTHFD2 (mouse): K50‑ac, MTHFD2 (rat): K49‑ac Characterization Methods used to characterize site in vivo:  mass spectrometry (in vitro)

K88-ac - MTHFD2 (human) ▲

Modsite: SHSYVLNkTRAAAVV SwissProt Entrez-Gene Orthologous residues MTHFD2 (human): K88‑ac, MTHFD2 (mouse): K88‑ac, MTHFD2 (rat): K87‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  293 (epithelial), HCT116 (intestinal), HeLa (cervical) Cellular systems studied:  cell lines Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes DEACETYLASE SIRT3 (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme, pharmacological inhibitor of upstream enzyme, modification-specific antibody, co-immunoprecipitation, transfection of inactive enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes cisplatin increase Downstream Regulation Effect of modification (function):  enzymatic activity, inhibited Associated Diseases Diseases Alterations Comments colorectal cancer decreased in colorectal cancer with high SIRT3 expression