Chin HG, et al. (2020) The microtubule-associated histone methyltransferase SET8, facilitated by transcription factor LSF, methylates α-tubulin. J Biol Chem 295, 4748-4759 32111740

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K311-me - TUBA1A (human) ▲

Modsite: kCDPRHGkyMACCLL SwissProt Entrez-Gene Orthologous residues TUBA1A (human): K311‑me, TUBA1A (mouse): K311‑me, TUBA1A (rat): K311‑me, TUBA1A (cow): K311‑me Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, microscopy-colocalization with upstream kinase, modification-specific antibody, mutation of modification site, western blotting Disease tissue studied:  hepatocellular carcinoma Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  green monkey, human Enzymes shown to modify site in vitro:  Type Enzyme METHYLTRANSFERASE SETD8 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes METHYLTRANSFERASE SETD8 (human) modification-specific antibody, pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes FQI1 decrease Downstream Regulation Effect of modification (function):  intracellular localization, molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays TFCP2 (human) Induces molecular association, regulation co-immunoprecipitation Comments:  TFCP2 recruits SETD8 to tubulin and enhances K311 methylation, while the inhibitor FQI1 inhibits TFCP2 methylation.