Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.7
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 34094857
Wang T, et al. (2021) Reduction of mechanical loading in tendons induces heterotopic ossification and activation of the β-catenin signaling pathway. J Orthop Translat 29, 42-50 34094857
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S33-p - CTNNB1 (rabbit)
Modsite: QQQSYLDsGIHSGAT SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): S33‑p, CTNNB1 (mouse): S33‑p, CTNNB1 (rat): S33‑p, CTNNB1 (rabbit): S33‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  stem
Cellular systems studied:  primary cells
Species studied:  rabbit
Comments:  tendon-derived stem cells (TDSCs) from rabbit has been used
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
mechanical stress decrease
Downstream Regulation
Effect of modification (function):  protein degradation
Effect of modification (process):  cell differentiation, inhibited

S552-p - CTNNB1 (rabbit)
Modsite: QDTQRRTsMGGTQQQ SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): S552‑p, CTNNB1 (mouse): S552‑p, CTNNB1 (rat): S552‑p, CTNNB1 (rabbit): S552‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  stem
Cellular systems studied:  primary cells
Species studied:  rabbit
Comments:  tendon-derived stem cells (TDSCs) from rabbit has been used
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
mechanical stress increase
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  cell differentiation, induced

S9-p - GSK3B (rabbit)
Modsite: SGRPRTTsFAESCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  stem
Cellular systems studied:  primary cells
Species studied:  rabbit
Comments:  tendon-derived stem cells (TDSCs) from rabbit has been used
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
mechanical stress decrease