Hu S, et al. (2021) The therapeutic effect of TBK1 in intervertebral disc degeneration via coordinating selective autophagy and autophagic functions. J Adv Res 30, 1-13 34026282

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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T266-p - SQSTM1 (rat) ▲

Modsite: GGKRSRLtPTsAESS SwissProt Entrez-Gene Orthologous residues SQSTM1 (human): T269‑p, SQSTM1 iso2 (human): T185‑p, SQSTM1 (mouse): T269‑p, SQSTM1 iso2 (mouse): T269‑p, SQSTM1 (rat): T266‑p, SQSTM1 iso2 (rat): T239‑p, SQSTM1 iso3 (rat): ‑ Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Cellular systems studied:  tissue Species studied:  human, rat Comments:  Nucleus pulposus cells (NPCs) Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes TNF no change compared to control

S269-p - SQSTM1 (rat) ▲

Modsite: RSRLtPTsAESSSTG SwissProt Entrez-Gene Orthologous residues SQSTM1 (human): S272‑p, SQSTM1 iso2 (human): S188‑p, SQSTM1 (mouse): T272‑p, SQSTM1 iso2 (mouse): T272‑p, SQSTM1 (rat): S269‑p, SQSTM1 iso2 (rat): S242‑p, SQSTM1 iso3 (rat): ‑ Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Cellular systems studied:  tissue Species studied:  human, rat Comments:  Nucleus pulposus cells (NPCs) Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes TNF no change compared to control

S348-p - SQSTM1 (rat) ▲

Modsite: SSKEVDPsTGELQsL SwissProt Entrez-Gene Orthologous residues SQSTM1 (human): S349‑p, SQSTM1 iso2 (human): S265‑p, SQSTM1 (mouse): S351‑p, SQSTM1 iso2 (mouse): S351‑p, SQSTM1 (rat): S348‑p, SQSTM1 iso2 (rat): S321‑p, SQSTM1 iso3 (rat): ‑ Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Cellular systems studied:  tissue Species studied:  human, rat Comments:  Nucleus pulposus cells (NPCs) Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes TNF increase MG132 TNF no effect upon treatment-induced increase

S402-p - SQSTM1 (rat) ▲

Modsite: ESLSQMLsMGFSDEG SwissProt Entrez-Gene Orthologous residues SQSTM1 (human): S403‑p, SQSTM1 iso2 (human): S319‑p, SQSTM1 (mouse): S405‑p, SQSTM1 iso2 (mouse): S367‑p, SQSTM1 (rat): S402‑p, SQSTM1 iso2 (rat): S375‑p, SQSTM1 iso3 (rat): ‑ Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Cellular systems studied:  tissue Species studied:  human, rat Comments:  Nucleus pulposus cells (NPCs) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE TBK1 (rat) transfection of wild-type enzyme, co-immunoprecipitation, microscopy-colocalization, phospho-antibody Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes TNF increase MG132 TNF augment treatment-induced increase okadaic_acid increase bafilomycin_A increase okadaic_acid, bafilomycin_A increase Downstream Regulation Effect of modification (process):  autophagy, induced