Curated Information
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Home > Curated Information Page > PubMed Id: 33514736
Vaughan CA, et al. (2021) The oncogenicity of tumor-derived mutant p53 is enhanced by the recruitment of PLK3. Nat Commun 12, 704 33514736
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S6-p - p53 (human)
Modsite: __MEEPQsDPSVEPP SwissProt Entrez-Gene
Orthologous residues
p53 (human): S6‑p, p53 iso2 (human): S6‑p, p53 iso4 (human): , p53 (mouse): S9‑p, p53 iso2 (mouse): S9‑p, p53 (rat): S6‑p, p53 (rabbit): S6‑p, p53 (green monkey): S6‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma
Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary), NCI-H1975 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human

S9-p - p53 (human)
Modsite: EEPQSDPsVEPPLSQ SwissProt Entrez-Gene
Orthologous residues
p53 (human): S9‑p, p53 iso2 (human): S9‑p, p53 iso4 (human): , p53 (mouse): S12‑p, p53 iso2 (mouse): S12‑p, p53 (rat): S9‑p, p53 (rabbit): S9‑p, p53 (green monkey): S9‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma
Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary), NCI-H1975 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human

S15-p - p53 (human)
Modsite: PSVEPPLsQETFSDL SwissProt Entrez-Gene
Orthologous residues
p53 (human): S15‑p, p53 iso2 (human): S15‑p, p53 iso4 (human): , p53 (mouse): S18‑p, p53 iso2 (mouse): S18‑p, p53 (rat): S15‑p, p53 (rabbit): S15‑p, p53 (green monkey): S15‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma
Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary), NCI-H1975 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human

S20-p - p53 (human)
Modsite: PLSQETFsDLWKLLP SwissProt Entrez-Gene
Orthologous residues
p53 (human): S20‑p, p53 iso2 (human): S20‑p, p53 iso4 (human): , p53 (mouse): S23‑p, p53 iso2 (mouse): S23‑p, p53 (rat): S20‑p, p53 (rabbit): S20‑p, p53 (green monkey): S20‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma
Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary), NCI-H1975 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PLK3 (human) siRNA inhibition of enzyme, genetic knockout/knockin of upstream enzyme, transfection of wild-type enzyme, co-immunoprecipitation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
siRNA decrease PLK3 siRNA
Downstream Regulation
Effect of modification (function):  activity, induced, molecular association, regulation
Effect of modification (process):  carcinogenesis, induced, cell growth, induced, transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
MED17 (human) Induces co-immunoprecipitation
Induces

S23-p - p53 (mouse)
Modsite: PLSQETFsGLWKLLP SwissProt Entrez-Gene
Orthologous residues
p53 (human): S20‑p, p53 iso2 (human): S20‑p, p53 iso4 (human): , p53 (mouse): S23‑p, p53 iso2 (mouse): S23‑p, p53 (rat): S20‑p, p53 (rabbit): S20‑p, p53 (green monkey): S20‑p