Zhang X, et al. (2020) The Inhibition Effects of Shenmai Injection on Acetylcholine-Induced Catecholamine Synthesis and Secretion by Modulating Nicotinic Acetylcholine Receptor Ion Channels in Cultured Bovine Adrenal Medullary Cells. Evid Based Complement Alternat Med 2020, 8514926 33456492

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S19-p - TH iso3 (human) ▲

Modsite: KGFRRAVsELDAKQA SwissProt Entrez-Gene Orthologous residues TH (human): S19‑p, TH iso2 (human): S19‑p, TH iso3 (human): S19‑p, TH iso4 (human): S19‑p, TH (mouse): S19‑p, TH (rat): S19‑p, TH (cow): S19‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  adrenal medulla Cellular systems studied:  tissue Species studied:  cow Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE CAMK2A (human) pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes ACh increase KN-93 ACh inhibit treatment-induced increase Shenmai ACh inhibit treatment-induced increase Shenmai decrease Shenmai ACh, KN-93 no effect upon treatment-induced decrease Downstream Regulation Effect of modification (function):  enzymatic activity, induced

S31-p - TH iso3 (human) ▲

Modsite: KQAEAIMsPRFIGRR SwissProt Entrez-Gene Orthologous residues TH (human): S62‑p, TH iso2 (human): S58‑p, TH iso3 (human): S31‑p, TH iso4 (human): S35‑p, TH (mouse): S31‑p, TH (rat): S31‑p, TH (cow): S31‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  adrenal medulla Cellular systems studied:  tissue Species studied:  cow Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes Shenmai no change compared to control

S40-p - TH iso3 (human) ▲

Modsite: RFIGRRQsLIEDARK SwissProt Entrez-Gene Orthologous residues TH (human): S71‑p, TH iso2 (human): S67‑p, TH iso3 (human): S40‑p, TH iso4 (human): S44‑p, TH (mouse): S40‑p, TH (rat): S40‑p, TH (cow): S40‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  adrenal medulla Cellular systems studied:  tissue Species studied:  cow Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKACA (human) pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes Shenmai decrease ACh increase Shenmai ACh inhibit treatment-induced increase H-89 ACh inhibit treatment-induced increase Shenmai ACh, H-89 no effect upon treatment-induced decrease Downstream Regulation Effect of modification (function):  enzymatic activity, induced