Fan G, et al. (2021) The ATM and ATR kinases regulate centrosome clustering and tumor recurrence by targeting KIFC1 phosphorylation. Nat Commun 12, 20 33397932

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S6-p - KIFC1 (human) ▲
Modsite: __MDPQRsPLLEVKG SwissProt Entrez-Gene Orthologous residues KIFC1 (human): S6‑p, KIFC1 (mouse): P8‑p, KIFC1 (rat): A32‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human

S26-p - KIFC1 (human) ▲

Modsite: RPLIkAPsQLPLsGs SwissProt Entrez-Gene Orthologous residues KIFC1 (human): S26‑p, KIFC1 (mouse): S28‑p, KIFC1 (rat): S52‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MCF-10A (breast cell), MDA-MB-231 (breast cell) Cellular systems studied:  cell lines, tissue Species studied:  human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE ATM (human) phospho-motif antibody, pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme, modification site within consensus motif, phospho-antibody, co-immunoprecipitation KINASE ATR (human) phospho-motif antibody, pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme, modification site within consensus motif, co-immunoprecipitation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes etoposide increase siRNA decrease ATM shRNA siRNA decrease ATR shRNA AZD6738 etoposide inhibit treatment-induced increase etoposide inhibit treatment-induced increase AZD1390 VE822 decrease camptothecin increase ionizing_radiation increase Downstream Regulation Effect of modification (function):  protein stabilization, ubiquitination Effect of modification (process):  carcinogenesis, induced, cell cycle regulation, chromatin organization, altered, DNA repair, induced Comments:  inhibits ubiquitination

S31-p - KIFC1 (human) ▲
Modsite: APsQLPLsGsRLKRR SwissProt Entrez-Gene Orthologous residues KIFC1 (human): S31‑p, KIFC1 (mouse): S33‑p, KIFC1 (rat): P57‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human

S33-p - KIFC1 (human) ▲
Modsite: sQLPLsGsRLKRRPD SwissProt Entrez-Gene Orthologous residues KIFC1 (human): S33‑p, KIFC1 (mouse): S35‑p, KIFC1 (rat): S59‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human

S71-p - KIFC1 (human) ▲
Modsite: TSHPRVPsLTTVPQT SwissProt Entrez-Gene Orthologous residues KIFC1 (human): S71‑p, KIFC1 (mouse): L73‑p, KIFC1 (rat): P97‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human

T326-p - KIFC1 (human) ▲

Modsite: PVLPGEPtPPPGLLL SwissProt Entrez-Gene Orthologous residues KIFC1 (human): T326‑p, KIFC1 (mouse): T327‑p, KIFC1 (rat): T346‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human

T346-p - KIFC1 (human) ▲

Modsite: GGPSDPPtRLSLSRS SwissProt Entrez-Gene Orthologous residues KIFC1 (human): T346‑p, KIFC1 (mouse): T347‑p, KIFC1 (rat): T366‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human