Curated Information
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Home > Curated Information Page > PubMed Id: 33206046
Li D, et al. (2020) Casein kinase 1G2 suppresses necroptosis-promoted testis aging by inhibiting receptor-interacting kinase 3. Elife 9 33206046
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S211-p - CK1G2 (mouse)
Modsite: IPYREHKsLTGtARY SwissProt Entrez-Gene
Orthologous residues
CK1G2 (human): S211‑p, CK1G2 (mouse): S211‑p, CK1G2 (rat): S211‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK1G2 (mouse)
Comments:  autophosphorylation
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
RIPK3 (human) Induces enzymatic activity, inhibited, molecular association, regulation, phosphorylation co-immunoprecipitation
Comments:  inhibits phosphorylation of MLKL, binding to RIPK3 inhibits RIPK3 binding to RIPK1

T215-p - CK1G2 (mouse)
Modsite: EHKsLTGtARYMSIN SwissProt Entrez-Gene
Orthologous residues
CK1G2 (human): T215‑p, CK1G2 (mouse): T215‑p, CK1G2 (rat): T215‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK1G2 (mouse)
Comments:  autophosphorylation
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
RIPK3 (human) Induces enzymatic activity, inhibited, phosphorylation, molecular association, regulation co-immunoprecipitation
Comments:  inhibits phosphorylation of MLKL, binding to RIPK3 inhibits RIPK3 binding to RIPK1

S345-p - MLKL (mouse)
Modsite: ELSKTQNsIsRtAKS SwissProt Entrez-Gene
Orthologous residues
MLKL (human): S358‑p, MLKL (mouse): S345‑p, MLKL iso2 (mouse): S345‑p, MLKL (rat): S345‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE RIPK3 (mouse) phospho-antibody, genetic knockout/knockin of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TRAIL, Z-VAD-FMK increase
TNF, Z-VAD-FMK increase
LPS, Z-VAD-FMK increase
LPS, Z-VAD-FMK CK1G2 (mouse) inhibit treatment-induced increase CK1G2 KO augments
TNF, Z-VAD-FMK CK1G2 (mouse) inhibit treatment-induced increase CK1G2 KO augments
TRAIL, Z-VAD-FMK CK1G2 (mouse) inhibit treatment-induced increase CK1G2 KO augments
development CK1G2 (mouse) increase CK1G2 KO
CK1G2 (mouse) decrease CK1G2 KO increases
RIPK3 (mouse) CK1G2 (mouse) inhibit treatment-induced decrease RIPK3 KO augments
development increase

T231-p - RIPK3 (mouse)
Modsite: EAELVDKtsLIRETV SwissProt Entrez-Gene
Orthologous residues
RIPK3 (human): P226‑p, RIPK3 (mouse): T231‑p, RIPK3 (rat): T228‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TRAIL, Z-VAD-FMK increase
TNF, Z-VAD-FMK increase
LPS, Z-VAD-FMK increase
LPS, Z-VAD-FMK CK1G2 (mouse) inhibit treatment-induced increase CK1G2 KO augments
TNF, Z-VAD-FMK CK1G2 (mouse) inhibit treatment-induced increase CK1G2 KO augments
TRAIL, Z-VAD-FMK CK1G2 (mouse) inhibit treatment-induced increase CK1G2 KO augments

S232-p - RIPK3 (mouse)
Modsite: AELVDKtsLIRETVC SwissProt Entrez-Gene
Orthologous residues
RIPK3 (human): S227‑p, RIPK3 (mouse): S232‑p, RIPK3 (rat): S229‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE RIPK3 (mouse)
Comments:  autophosphorylation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CK1D (mouse) decrease
CK1E (mouse) decrease
CK1G2 (mouse) decrease
CK1A (mouse) no change compared to control
CK1G1 (mouse) no change compared to control
CK2A1 (mouse) no change compared to control
CK2A2 (mouse) no change compared to control
CK2B (mouse) no change compared to control
TRAIL, Z-VAD-FMK increase
TNF, Z-VAD-FMK increase
LPS, Z-VAD-FMK increase
LPS, Z-VAD-FMK CK1G2 (mouse) inhibit treatment-induced increase CK1G2 KO augments
TNF, Z-VAD-FMK CK1G2 (mouse) inhibit treatment-induced increase CK1G2 KO augments
TRAIL, Z-VAD-FMK CK1G2 (mouse) inhibit treatment-induced increase CK1G2 KO augments
Downstream Regulation
Effect of modification (function):  phosphorylation
Comments:  induces phosphorylation of MLKL