Zhao X, et al. (2020) PKCε SUMOylation Is Required for Mediating the Nociceptive Signaling of Inflammatory Pain. Cell Rep 33, 108191 33027667

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K534-sm - PKCE (human) ▲

Modsite: GVIYRDLkLDNILLD SwissProt Entrez-Gene Orthologous residues PKCE (human): K534‑sm, PKCE (mouse): K534‑sm, PKCE (rat): K534‑sm Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  CHO-K1 (fibroblast), HeLa (cervical), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster, human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes DESUMOYLASE SENP1 (human) co-immunoprecipitation, transfection of wild-type enzyme, transfection of inactive enzyme SUMO LIGASE UBC9 (human) co-immunoprecipitation, transfection of wild-type enzyme Phosphorylation positively affects PKCE binding to UBC9. KINASE PKCE (human) siRNA inhibition of enzyme, phospho-antibody, co-immunoprecipitation SUMO1 works through PKCe to promote binding and enhance the phosphorylation of TRPV1 S800 Downstream Regulation Effect of modification (function):  enzymatic activity, induced, sumoylation Comments:  K534 sumoylation plays a critical role in the development of in¿ammatory thermal hyperalgesia, PKCE phosphorylation is required for it's sumoylation

S729-p - PKCE (human) ▲

Modsite: QEEFKGFsYFGEDLM SwissProt Entrez-Gene Orthologous residues PKCE (human): S729‑p, PKCE (mouse): S729‑p, PKCE (rat): S729‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  CHO-K1 (fibroblast), HeLa (cervical), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster, human, mouse Downstream Regulation Effect of modification (function):  sumoylation Comments:  PKCE phosphorylation is required for it's sumoylation

S502-p - TRPV1 (human) ▲

Modsite: YFLQRRPsMKTLFVD SwissProt Entrez-Gene Orthologous residues TRPV1 (human): S502‑p, TRPV1 (mouse): S503‑p, TRPV1 (rat): S502‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  CHO-K1 (fibroblast), HeLa (cervical), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster, human, mouse

S801-p - TRPV1 (human) ▲

Modsite: VPLLREAsARDRQsA SwissProt Entrez-Gene Orthologous residues TRPV1 (human): S801‑p, TRPV1 (mouse): S801‑p, TRPV1 (rat): S800‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  CHO-K1 (fibroblast), HeLa (cervical), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster, human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKCE (human) siRNA inhibition of enzyme, phospho-antibody, co-immunoprecipitation SUMO1 works through PKCe to promote binding and enhance the phosphorylation of TRPV1 S800