Curated Information
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Home > Curated Information Page > PubMed Id: 33027667
Zhao X, et al. (2020) PKCĪµ SUMOylation Is Required for Mediating the Nociceptive Signaling of Inflammatory Pain. Cell Rep 33, 108191 33027667
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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K534-sm - PKCE (human)
Modsite: GVIYRDLkLDNILLD SwissProt Entrez-Gene
Orthologous residues
PKCE (human): K534‑sm, PKCE (mouse): K534‑sm, PKCE (rat): K534‑sm
Characterization
Methods used to characterize site in vivo immunoprecipitation, modification-specific antibody, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  CHO-K1 (fibroblast), HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster, human, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DESUMOYLASE SENP1 (human) co-immunoprecipitation, transfection of wild-type enzyme, transfection of inactive enzyme
SUMO LIGASE UBC9 (human) co-immunoprecipitation, transfection of wild-type enzyme Phosphorylation positively affects PKCE binding to UBC9.
KINASE PKCE (human) siRNA inhibition of enzyme, phospho-antibody, co-immunoprecipitation SUMO1 works through PKCe to promote binding and enhance the phosphorylation of TRPV1 S800
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, sumoylation
Comments:  K534 sumoylation plays a critical role in the development of in¿ammatory thermal hyperalgesia, PKCE phosphorylation is required for it's sumoylation

S729-p - PKCE (human)
Modsite: QEEFKGFsYFGEDLM SwissProt Entrez-Gene
Orthologous residues
PKCE (human): S729‑p, PKCE (mouse): S729‑p, PKCE (rat): S729‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, modification-specific antibody, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  CHO-K1 (fibroblast), HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster, human, mouse
Downstream Regulation
Effect of modification (function):  sumoylation
Comments:  PKCE phosphorylation is required for it's sumoylation

S502-p - TRPV1 (human)
Modsite: YFLQRRPsMKTLFVD SwissProt Entrez-Gene
Orthologous residues
TRPV1 (human): S502‑p, TRPV1 (mouse): S503‑p, TRPV1 (rat): S502‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, modification-specific antibody, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  CHO-K1 (fibroblast), HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster, human, mouse

S801-p - TRPV1 (human)
Modsite: VPLLREAsARDRQsA SwissProt Entrez-Gene
Orthologous residues
TRPV1 (human): S801‑p, TRPV1 (mouse): S801‑p, TRPV1 (rat): S800‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, modification-specific antibody, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  CHO-K1 (fibroblast), HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster, human, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCE (human) siRNA inhibition of enzyme, phospho-antibody, co-immunoprecipitation SUMO1 works through PKCe to promote binding and enhance the phosphorylation of TRPV1 S800