Santio NM, et al. (2020) PIM1 accelerates prostate cancer cell motility by phosphorylating actin capping proteins. Cell Commun Signal 18, 121 32771000

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S106-p - CAPZA1 (human) ▲

Modsite: DHLRkEAsDPQPEEA SwissProt Entrez-Gene Orthologous residues CAPZA1 (human): S106‑p, CAPZA1 (mouse): S106‑p, CAPZA1 (rat): S106‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mass spectrometry (in vitro), mutation of modification site Disease tissue studied:  prostate cancer Relevant cell lines - cell types - tissues:  DU 145 (prostate cell), LNCaP (prostate cell), PC3 (prostate cell) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE Pim1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Pim1 (human) pharmacological inhibitor of upstream enzyme, microscopy-colocalization, co-immunoprecipitation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes AZD1208 decrease SGI-1776 decrease Downstream Regulation Effect of modification (process):  cell adhesion, induced, cell motility, induced, cytoskeletal reorganization

S126-p - CAPZA1 (human) ▲

Modsite: SWREsCDsALRAYVk SwissProt Entrez-Gene Orthologous residues CAPZA1 (human): S126‑p, CAPZA1 (mouse): S126‑p, CAPZA1 (rat): S126‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mass spectrometry (in vitro), mutation of modification site Disease tissue studied:  prostate cancer Relevant cell lines - cell types - tissues:  DU 145 (prostate cell), LNCaP (prostate cell), PC3 (prostate cell) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE Pim1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Pim1 (human) pharmacological inhibitor of upstream enzyme, microscopy-colocalization, co-immunoprecipitation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes AZD1208 decrease SGI-1776 decrease Downstream Regulation Effect of modification (process):  cell adhesion, induced, cell motility, induced, cytoskeletal reorganization

T186-p - CAPZB iso1 (human) ▲

Modsite: tNKsGsGtMNLGGsL SwissProt Entrez-Gene Orthologous residues CAPZB (human): T186‑p, CAPZB iso1 (human): T186‑p, CAPZB (mouse): T186‑p, CAPZB iso2 (mouse): T186‑p, CAPZB (rat): T186‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mass spectrometry (in vitro), mutation of modification site Disease tissue studied:  prostate cancer Relevant cell lines - cell types - tissues:  DU 145 (prostate cell), LNCaP (prostate cell), PC3 (prostate cell) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE Pim1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Pim1 (human) pharmacological inhibitor of upstream enzyme, microscopy-colocalization, co-immunoprecipitation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes AZD1208 decrease SGI-1776 decrease Downstream Regulation Effect of modification (process):  cell adhesion, induced, cell motility, induced, cytoskeletal reorganization

S192-p - CAPZB iso1 (human) ▲

Modsite: GtMNLGGsLtRQMEk SwissProt Entrez-Gene Orthologous residues CAPZB (human): S192‑p, CAPZB iso1 (human): S192‑p, CAPZB (mouse): S192‑p, CAPZB iso2 (mouse): S192‑p, CAPZB (rat): S192‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mass spectrometry (in vitro), mutation of modification site Disease tissue studied:  prostate cancer Relevant cell lines - cell types - tissues:  DU 145 (prostate cell), LNCaP (prostate cell), PC3 (prostate cell) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE Pim1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Pim1 (human) pharmacological inhibitor of upstream enzyme, microscopy-colocalization, co-immunoprecipitation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes AZD1208 decrease SGI-1776 decrease Downstream Regulation Effect of modification (process):  cell adhesion, induced, cell motility, induced, cytoskeletal reorganization

S226-p - CAPZB iso1 (human) ▲

Modsite: DMENkIRsTLNEIyF SwissProt Entrez-Gene Orthologous residues CAPZB (human): S226‑p, CAPZB iso1 (human): S226‑p, CAPZB (mouse): S226‑p, CAPZB iso2 (mouse): S226‑p, CAPZB (rat): S226‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mass spectrometry (in vitro), mutation of modification site Disease tissue studied:  prostate cancer Relevant cell lines - cell types - tissues:  DU 145 (prostate cell), LNCaP (prostate cell), PC3 (prostate cell) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE Pim1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Pim1 (human) pharmacological inhibitor of upstream enzyme, microscopy-colocalization, co-immunoprecipitation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes AZD1208 decrease SGI-1776 decrease Downstream Regulation Effect of modification (process):  cell adhesion, induced, cell motility, induced, cytoskeletal reorganization