Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.6
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 32757223
Hegde RN, et al. (2020) TBK1 phosphorylates mutant Huntingtin and suppresses its aggregation and toxicity in Huntington's disease models. EMBO J 39, e104671 32757223
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

T3-p - Huntingtin (human)
Modsite: _____MAtLEkLMkA SwissProt Entrez-Gene
Orthologous residues
Huntingtin (human): T3‑p, Huntingtin (mouse): T3‑p, Huntingtin (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  C. elegans, human

S13-p - Huntingtin (human)
Modsite: kLMkAFEsLksFQQQ SwissProt Entrez-Gene
Orthologous residues
Huntingtin (human): S13‑p, Huntingtin (mouse): S13‑p, Huntingtin (rat): S6‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, striatal'-brain, 293 (epithelial), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  C. elegans, human, rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TBK1 (human)
KINASE IKKB (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKKB (human) transfection of wild-type enzyme
KINASE IKKE (human) siRNA inhibition of enzyme
KINASE TBK1 (human) transfection of inactive enzyme, co-immunoprecipitation, transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
siRNA decrease IKKE siRNA
Downstream Regulation
Effect of modification (function):  intracellular localization
Associated Diseases
Diseases Alterations Comments
Huntington's disease decreased

S16-p - Huntingtin (human)
Modsite: kAFEsLksFQQQQQQ SwissProt Entrez-Gene
Orthologous residues
Huntingtin (human): S16‑p, Huntingtin (mouse): S16‑p, Huntingtin (rat): S9‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE IKKB (human)
KINASE TBK1 (human)