Curated Information
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Home > Curated Information Page > PubMed Id: 32491969
Zhang Q, et al. (2020) Multisite Phosphorylation Determines the Formation of Ska-Ndc80 Macro-complexes That Are Essential for Chromosome Segregation during Mitosis. Mol Biol Cell, mbcE19100569 32491969
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T203-p - SKA3 (human)
Modsite: sLVkVLKtPKCALkM SwissProt Entrez-Gene
Orthologous residues
SKA3 (human): T203‑p, SKA3 iso3 (human): T203‑p, SKA3 (mouse): T202‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, immunoassay, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Effect of modification (process):  cell cycle regulation, chromatin organization, altered, cytoskeletal reorganization
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HEC1 (human) Induces pull-down assay

T217-p - SKA3 (human)
Modsite: MDDFECVtPKLEHFG SwissProt Entrez-Gene
Orthologous residues
SKA3 (human): T217‑p, SKA3 iso3 (human): T217‑p, SKA3 (mouse): T216‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, immunoassay, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Effect of modification (process):  cell cycle regulation, chromatin organization, altered, cytoskeletal reorganization
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HEC1 (human) Induces pull-down assay

S283-p - SKA3 (human)
Modsite: sDAEytNsPLVPTFC SwissProt Entrez-Gene
Orthologous residues
SKA3 (human): S283‑p, SKA3 iso3 (human): S283‑p, SKA3 (mouse): S289‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, immunoassay, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Effect of modification (process):  cell cycle regulation, chromatin organization, altered, cytoskeletal reorganization
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HEC1 (human) Induces pull-down assay

T291-p - SKA3 (human)
Modsite: PLVPTFCtPGLkIPS SwissProt Entrez-Gene
Orthologous residues
SKA3 (human): T291‑p, SKA3 iso3 (human): T291‑p, SKA3 (mouse): T297‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, immunoassay, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Effect of modification (process):  cell cycle regulation, chromatin organization, altered, cytoskeletal reorganization
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HEC1 (human) Induces pull-down assay

T358-p - SKA3 (human)
Modsite: SYENLLRtPtPPEVT SwissProt Entrez-Gene
Orthologous residues
SKA3 (human): T358‑p, SKA3 iso3 (human): T358‑p, SKA3 (mouse): T363‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, immunoassay, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Effect of modification (process):  cell cycle regulation, chromatin organization, altered, cytoskeletal reorganization
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HEC1 (human) Induces pull-down assay

T360-p - SKA3 (human)
Modsite: ENLLRtPtPPEVTkI SwissProt Entrez-Gene
Orthologous residues
SKA3 (human): T360‑p, SKA3 iso3 (human): T360‑p, SKA3 (mouse): S365‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, immunoassay, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Effect of modification (process):  cell cycle regulation, chromatin organization, altered, cytoskeletal reorganization
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HEC1 (human) Induces pull-down assay