Curated Information
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Home > Curated Information Page > PubMed Id: 32294430
Romero-Pozuelo J, et al. (2020) Cdk4 and Cdk6 Couple the Cell-Cycle Machinery to Cell Growth via mTORC1. Cell Rep 31, 107504 32294430
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S664-p - TSC2 (human)
Modsite: KkTSGPLsPPTGPPG SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S664‑p, TSC2 iso2 (human): S664‑p, TSC2 iso3 (human): S664‑p, TSC2 iso4 (human): S664‑p, TSC2 (mouse): S664‑p, TSC2 iso6 (mouse): S664‑p, TSC2 (rat): S664‑p, TSC2 iso2 (rat): S664‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S1217-p - TSC2 (human)
Modsite: MSLENPLsPFSSDIN SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1217‑p

S1452-p - TSC2 (human)
Modsite: LPSssPRsPsGLRPR SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1452‑p, TSC2 iso2 (human): S1409‑p, TSC2 iso3 (human): S1408‑p, TSC2 iso4 (human): S1429‑p, TSC2 (mouse): S1455‑p, TSC2 iso6 (mouse): S1433‑p, TSC2 (rat): S1456‑p, TSC2 iso2 (rat): S1413‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  glioblastoma multiforme, glioma, breast cancer
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MCF-7 (breast cell), MDA-MB-453 (breast cell), MEF (fibroblast), T98G (glial)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK4 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK4 (human) modification site within consensus motif, pharmacological inhibitor of upstream enzyme, phospho-motif antibody, siRNA inhibition of enzyme, co-immunoprecipitation
KINASE CDK6 (human) modification site within consensus motif, pharmacological inhibitor of upstream enzyme, phospho-motif antibody, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
amino_acids no change compared to control
Downstream Regulation
Effect of modification (function):  activity, inhibited, phosphorylation
Effect of modification (process):  cell cycle regulation, cell growth, induced, translation, induced
Comments:  induces S6K phosphorylation