Curated Information
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Home > Curated Information Page > PubMed Id: 21325048
Langlais P, Yi Z, Mandarino LJ (2011) The Identification of Raptor as a Substrate for p44/42 MAPK. Endocrinology 152, 1264-73 21325048
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T412-p - p70S6K (human)
Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin Raptor (human) inhibit treatment-induced increase raptor S696A mutant

Y692-p - Raptor (human)
Modsite: FIEEEKNyALPsPAt SwissProt Entrez-Gene
Orthologous residues
Raptor (human): Y692‑p, Raptor (mouse): Y692‑p, Raptor (rat): Y692‑p

S696-p - Raptor (human)
Modsite: EKNyALPsPAttEGG SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S696‑p, Raptor (mouse): S696‑p, Raptor (rat): S696‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  CHO (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
wortmannin insulin inhibit treatment-induced increase
PD98059 insulin inhibit treatment-induced increase
rapamycin no change compared to control
Downstream Regulation
Effect of modification (function):  phosphorylation
Comments:  mutation of S696A interferes with the insulin-stimulated phosphorylation of p70S6K

T699-p - Raptor (human)
Modsite: yALPsPAttEGGsLt SwissProt Entrez-Gene
Orthologous residues
Raptor (human): T699‑p, Raptor (mouse): A699‑p, Raptor (rat): A699‑p

T700-p - Raptor (human)
Modsite: ALPsPAttEGGsLtP SwissProt Entrez-Gene
Orthologous residues
Raptor (human): T700‑p, Raptor (mouse): T700‑p, Raptor (rat): T700‑p

S704-p - Raptor (human)
Modsite: PAttEGGsLtPVRDs SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S704‑p, Raptor (mouse): S704‑p, Raptor (rat): S704‑p

T706-p - Raptor (human)
Modsite: ttEGGsLtPVRDsPC SwissProt Entrez-Gene
Orthologous residues
Raptor (human): T706‑p, Raptor (mouse): T706‑p, Raptor (rat): T706‑p

S721-p - Raptor (human)
Modsite: tPrLrsVssyGNIRA SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S721‑p, Raptor (mouse): S721‑p, Raptor (rat): S721‑p

S722-p - Raptor (human)
Modsite: PrLrsVssyGNIRAV SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S722‑p, Raptor (mouse): S722‑p, Raptor (rat): S722‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin decrease
wortmannin insulin inhibit treatment-induced decrease
PD98059 insulin inhibit treatment-induced decrease
rapamycin no change compared to control

S854-p - Raptor (human)
Modsite: PQRVLDtssLtQsAP SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S854‑p, Raptor (mouse): S854‑p, Raptor (rat): S854‑p

S855-p - Raptor (human)
Modsite: QRVLDtssLtQsAPA SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S855‑p, Raptor (mouse): S855‑p, Raptor (rat): S855‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
wortmannin insulin inhibit treatment-induced increase
PD98059 insulin inhibit treatment-induced increase
rapamycin no change compared to control

T857-p - Raptor (human)
Modsite: VLDtssLtQsAPAsP SwissProt Entrez-Gene
Orthologous residues
Raptor (human): T857‑p, Raptor (mouse): T857‑p, Raptor (rat): T857‑p

S859-p - Raptor (human)
Modsite: DtssLtQsAPAsPtN SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S859‑p, Raptor (mouse): S859‑p, Raptor (rat): S859‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control
rapamycin decrease

S863-p - Raptor (human)
Modsite: LtQsAPAsPtNkGVH SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S863‑p, Raptor (mouse): S863‑p, Raptor (rat): S863‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
wortmannin insulin inhibit treatment-induced increase
PD98059 insulin inhibit treatment-induced increase
rapamycin decrease

T865-p - Raptor (human)
Modsite: QsAPAsPtNkGVHIH SwissProt Entrez-Gene
Orthologous residues
Raptor (human): T865‑p, Raptor (mouse): T865‑p, Raptor (rat): T865‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
wortmannin insulin inhibit treatment-induced increase
PD98059 insulin inhibit treatment-induced increase
rapamycin decrease

S877-p - Raptor (human)
Modsite: HIHQAGGsPPAssts SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S877‑p, Raptor (mouse): S877‑p, Raptor (rat): S877‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control
rapamycin no change compared to control

S881-p - Raptor (human)
Modsite: AGGsPPAsstsSssL SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S881‑p, Raptor (mouse): S881‑p, Raptor (rat): S881‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control
rapamycin no change compared to control

S882-p - Raptor (human)
Modsite: GGsPPAsstsSssLt SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S882‑p, Raptor (mouse): S882‑p, Raptor (rat): S882‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control
rapamycin no change compared to control

T883-p - Raptor (human)
Modsite: GsPPAsstsSssLtN SwissProt Entrez-Gene
Orthologous residues
Raptor (human): T883‑p, Raptor (mouse): T883‑p, Raptor (rat): T883‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control
rapamycin no change compared to control

S884-p - Raptor (human)
Modsite: sPPAsstsSssLtND SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S884‑p, Raptor (mouse): S884‑p, Raptor (rat): S884‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control
rapamycin no change compared to control

S886-p - Raptor (human)
Modsite: PAsstsSssLtNDVA SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S886‑p, Raptor (mouse): C886‑p, Raptor (rat): C886‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control
rapamycin no change compared to control

S887-p - Raptor (human)
Modsite: AsstsSssLtNDVAk SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S887‑p, Raptor (mouse): S887‑p, Raptor (rat): S887‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control
rapamycin no change compared to control

T889-p - Raptor (human)
Modsite: stsSssLtNDVAkQP SwissProt Entrez-Gene
Orthologous residues
Raptor (human): T889‑p, Raptor (mouse): T889‑p, Raptor (rat): T889‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control
rapamycin no change compared to control