Curated Information
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Home > Curated Information Page > PubMed Id: 21310943
Doller A, et al. (2011) High-constitutive HuR phosphorylation at Ser 318 by PKC{delta} propagates tumor relevant functions in colon carcinoma cells. Carcinogenesis 32, 676-85 21310943
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S318-p - HuR (human)
Modsite: GDkILQVsFkTNkSH SwissProt Entrez-Gene
Orthologous residues
HuR (human): S318‑p, HuR iso2 (human): S345‑p, HuR (mouse): S318‑p, HuR (rat): S318‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  CCD 841, colon, DLD1 (intestinal)
Cellular systems studied:  cell lines, tissue
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCD (human) activation of upstream enzyme, pharmacological inhibitor of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
rottlerin decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  carcinogenesis, altered, cell cycle regulation, cell motility, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Induces
Comments:  increases cell migration and proliferation
Associated Diseases
Diseases Alterations Comments
colorectal carcinoma increased

T507-p - PKCD (human)
Modsite: FGEsRAstFCGtPDy SwissProt Entrez-Gene
Orthologous residues
PKCD (human): T507‑p, PKCD iso2 (human): T538‑p, PKCD (mouse): T505‑p, PKCD iso2 (mouse): T531‑p, PKCD (rat): T505‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  Caco-2 (intestinal), CCD 841, DLD1 (intestinal), HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human