Curated Information
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Home > Curated Information Page > PubMed Id: 12501214
Greif DM, Kou R, Michel T (2002) Site-specific dephosphorylation of endothelial nitric oxide synthase by protein phosphatase 2A: evidence for crosstalk between phosphorylation sites. Biochemistry 41, 15845-53 12501214
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S116-p - eNOS (cow)
Modsite: RKLQTRPsPGPPPAE SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S114‑p, eNOS (mouse): T113‑p, eNOS (rat): T113‑p, eNOS (rabbit): T120‑p, eNOS (pig): S116‑p, eNOS (cow): S116‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  cow, green monkey
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP2CA (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
calyculin_A increase
okadaic_acid increase
ciclosporin no change compared to control
VEGF calyculin_A inhibit treatment-induced increase
VEGF inhibit treatment-induced increase

T497-p - eNOS (cow)
Modsite: AGITRKktFKEVANA SwissProt Entrez-Gene
Orthologous residues
eNOS (human): T495‑p, eNOS (mouse): T494‑p, eNOS (rat): T494‑p, eNOS (rabbit): T501‑p, eNOS (pig): T497‑p, eNOS (cow): T497‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  cow, green monkey
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP2CA (human)

S1179-p - eNOS (cow)
Modsite: TSRIRtQsFsLQERH SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S1177‑p, eNOS (mouse): S1176‑p, eNOS (rat): S1176‑p, eNOS (rabbit): S1183‑p, eNOS (pig): S1179‑p, eNOS (cow): S1179‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  cow, green monkey
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP2CA (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
calyculin_A increase
okadaic_acid increase
ciclosporin no change compared to control
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced