Curated Information
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Home > Curated Information Page > PubMed Id: 12171920
Michell BJ, et al. (2002) Identification of regulatory sites of phosphorylation of the bovine endothelial nitric-oxide synthase at serine 617 and serine 635. J Biol Chem 277, 42344-51 12171920
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S615-p - eNOS (human)
Modsite: SYKIRFNsISCSDPL SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S615‑p, eNOS (mouse): S614‑p, eNOS (rat): S614‑p, eNOS (rabbit): S621‑p, eNOS (pig): S617‑p, eNOS (cow): S617‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
Relevant cell lines - cell types - tissues:  BAE
Cellular systems studied:  cell lines
Species studied:  cow
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
KINASE PKACA (human)

S633-p - eNOS (human)
Modsite: WRRKRKEssNtDsAG SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S633‑p, eNOS (mouse): S632‑p, eNOS (rat): S632‑p, eNOS (rabbit): S639‑p, eNOS (pig): S635‑p, eNOS (cow): S635‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
Relevant cell lines - cell types - tissues:  BAE
Cellular systems studied:  cell lines
Species studied:  cow
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKACA (human)

S1177-p - eNOS (human)
Modsite: TSRIRtQsFsLQERQ SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S1177‑p, eNOS (mouse): S1176‑p, eNOS (rat): S1176‑p, eNOS (rabbit): S1183‑p, eNOS (pig): S1179‑p, eNOS (cow): S1179‑p

S614-p - eNOS (rat)
Modsite: SYKIRFNsVSCSDPL SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S615‑p, eNOS (mouse): S614‑p, eNOS (rat): S614‑p, eNOS (rabbit): S621‑p, eNOS (pig): S617‑p, eNOS (cow): S617‑p

S632-p - eNOS (rat)
Modsite: WRRKRKEsSNTDSAG SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S633‑p, eNOS (mouse): S632‑p, eNOS (rat): S632‑p, eNOS (rabbit): S639‑p, eNOS (pig): S635‑p, eNOS (cow): S635‑p

S1176-p - eNOS (rat)
Modsite: TSRIRtQsFsLQERQ SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S1177‑p, eNOS (mouse): S1176‑p, eNOS (rat): S1176‑p, eNOS (rabbit): S1183‑p, eNOS (pig): S1179‑p, eNOS (cow): S1179‑p

S617-p - eNOS (cow)
Modsite: SYKIRFNsVSCSDPL SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S615‑p, eNOS (mouse): S614‑p, eNOS (rat): S614‑p, eNOS (rabbit): S621‑p, eNOS (pig): S617‑p, eNOS (cow): S617‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
Relevant cell lines - cell types - tissues:  BAE
Cellular systems studied:  cell lines
Species studied:  cow
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bradykinin increase
LY294002 bradykinin inhibit treatment-induced increase
BAPTA-AM bradykinin inhibit treatment-induced increase
wortmannin bradykinin inhibit treatment-induced increase
VEGF increase
ATP increase
IBMX no change compared to control
KT5720 bradykinin no effect upon treatment-induced increase

S635-p - eNOS (cow)
Modsite: WRRKRKEsSNTDSAG SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S633‑p, eNOS (mouse): S632‑p, eNOS (rat): S632‑p, eNOS (rabbit): S639‑p, eNOS (pig): S635‑p, eNOS (cow): S635‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
Relevant cell lines - cell types - tissues:  BAE
Cellular systems studied:  cell lines
Species studied:  cow
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bradykinin increase
LY294002 bradykinin no effect upon treatment-induced increase
wortmannin bradykinin no effect upon treatment-induced increase
KT5720 bradykinin inhibit treatment-induced increase
BAPTA-AM bradykinin no effect upon treatment-induced increase
VEGF increase
ATP increase
IBMX increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced