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Home > Curated Information Page > PubMed Id: 28325821
Uhm M, et al. (2017) Phosphorylation of the exocyst protein Exo84 by TBK1 promotes insulin-stimulated GLUT4 trafficking. Sci Signal 10 28325821
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S172-p - TBK1 (human)
Modsite: EDDEQFVsLyGTEEy SwissProt Entrez-Gene
Orthologous residues
TBK1 (human): S172‑p, TBK1 (mouse): S172‑p, TBK1 (rat): S172‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase
insulin no change compared to control

S28-p - EXOC8 (rat)
Modsite: RLYVKQLsQQsDGDR SwissProt Entrez-Gene
Orthologous residues
EXOC8 (human): S32‑p, EXOC8 (mouse): S28‑p, EXOC8 (rat): S28‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  '3T3-L1, differentiated' (adipocyte), COS (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TBK1 (rat)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  exocytosis, inhibited
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
EXOC8 (rat) Disrupts co-immunoprecipitation
Sec5 (rat) Disrupts co-immunoprecipitation

S31-p - EXOC8 (rat)
Modsite: VKQLsQQsDGDRDLQ SwissProt Entrez-Gene
Orthologous residues
EXOC8 (human): S35‑p, EXOC8 (mouse): S31‑p, EXOC8 (rat): S31‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  '3T3-L1, differentiated' (adipocyte), COS (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TBK1 (rat)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  exocytosis, inhibited
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Sec5 (rat) Disrupts co-immunoprecipitation
EXOC8 (rat) Disrupts co-immunoprecipitation

S92-p - EXOC8 (rat)
Modsite: HLLTEQKssLESIPL SwissProt Entrez-Gene
Orthologous residues
EXOC8 (human): S96‑p, EXOC8 (mouse): S92‑p, EXOC8 (rat): S92‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  '3T3-L1, differentiated' (adipocyte), COS (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TBK1 (rat)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  exocytosis, inhibited
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Sec5 (rat) Disrupts co-immunoprecipitation
EXOC8 (rat) Disrupts co-immunoprecipitation

S93-p - EXOC8 (rat)
Modsite: LLTEQKssLESIPLA SwissProt Entrez-Gene
Orthologous residues
EXOC8 (human): S97‑p, EXOC8 (mouse): S93‑p, EXOC8 (rat): S93‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  '3T3-L1, differentiated' (adipocyte), COS (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TBK1 (rat)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  exocytosis, inhibited
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Sec5 (rat) Disrupts co-immunoprecipitation
EXOC8 (rat) Disrupts co-immunoprecipitation

T152-p - EXOC8 (rat)
Modsite: GEEGKQRtLTTLLEK SwissProt Entrez-Gene
Orthologous residues
EXOC8 (human): T161‑p, EXOC8 (mouse): T152‑p, EXOC8 (rat): T152‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  '3T3-L1, differentiated' (adipocyte), COS (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TBK1 (rat)

S276-p - EXOC8 (rat)
Modsite: EETKRALsDKRRREQ SwissProt Entrez-Gene
Orthologous residues
EXOC8 (human): S285‑p, EXOC8 (mouse): S276‑p, EXOC8 (rat): S276‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  '3T3-L1, differentiated' (adipocyte), COS (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TBK1 (rat)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  exocytosis, inhibited
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
EXOC8 (rat) Disrupts co-immunoprecipitation
Sec5 (rat) Disrupts co-immunoprecipitation

T313-p - EXOC8 (rat)
Modsite: EAEEELAtPEAEEEK SwissProt Entrez-Gene
Orthologous residues
EXOC8 (human): V322‑p, EXOC8 (mouse): T313‑p, EXOC8 (rat): T313‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  '3T3-L1, differentiated' (adipocyte), COS (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TBK1 (rat)

S366-p - EXOC8 (rat)
Modsite: EDKPSPPsVKELRAK SwissProt Entrez-Gene
Orthologous residues
EXOC8 (human): P375‑p, EXOC8 (mouse): P366‑p, EXOC8 (rat): S366‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  '3T3-L1, differentiated' (adipocyte), COS (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TBK1 (rat)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  exocytosis, inhibited
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Sec5 (rat) Disrupts co-immunoprecipitation
EXOC8 (rat) Disrupts co-immunoprecipitation

S505-p - EXOC8 (rat)
Modsite: QVFDSKEsLSTAAEC SwissProt Entrez-Gene
Orthologous residues
EXOC8 (human): S514‑p, EXOC8 (mouse): S505‑p, EXOC8 (rat): S505‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  '3T3-L1, differentiated' (adipocyte), COS (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TBK1 (rat)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  exocytosis, inhibited
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Sec5 (rat) Disrupts co-immunoprecipitation
EXOC8 (rat) Disrupts co-immunoprecipitation

S670-p - EXOC8 (rat)
Modsite: TFIRQNAsFLyDTVL SwissProt Entrez-Gene
Orthologous residues
EXOC8 (human): S679‑p, EXOC8 (mouse): S670‑p, EXOC8 (rat): S670‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  '3T3-L1, differentiated' (adipocyte), COS (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TBK1 (rat)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  exocytosis, inhibited
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Sec5 (rat) Disrupts co-immunoprecipitation
EXOC8 (rat) Disrupts co-immunoprecipitation