Haycock JW, Ahn NG, Cobb MH, Krebs EG (1992) ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ. Proc Natl Acad Sci U S A 89, 2365-9 1347949

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S19-p - TH (rat) ▲

Modsite: KGFRRAVsEQDAKQA SwissProt Entrez-Gene Orthologous residues TH (human): S19‑p, TH iso2 (human): S19‑p, TH iso3 (human): S19‑p, TH iso4 (human): S19‑p, TH (mouse): S19‑p, TH (rat): S19‑p, TH (cow): S19‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes Ba(2+) increase bradykinin increase genistein bradykinin no effect upon treatment-induced increase

S31-p - TH (rat) ▲

Modsite: KQAEAVTsPRFIGRR SwissProt Entrez-Gene Orthologous residues TH (human): S62‑p, TH iso2 (human): S58‑p, TH iso3 (human): S31‑p, TH iso4 (human): S35‑p, TH (mouse): S31‑p, TH (rat): S31‑p, TH (cow): S31‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping Cellular systems studied:  cell lines Species studied:  rat Enzymes shown to modify site in vitro:  Type Enzyme KINASE ERK1 (mouse) KINASE ERK1 (rat) KINASE ERK2 (rat) KINASE ERK2 (mouse) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE ERK2 (rat) pharmacological activator of upstream enzyme KINASE ERK1 (rat) pharmacological activator of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes bradykinin increase genistein bradykinin inhibit treatment-induced increase muscarine increase genistein muscarine inhibit treatment-induced increase NGF increase genistein NGF no effect upon treatment-induced increase Ba(2+) increase genistein Ba(2+) inhibit treatment-induced increase phorbol_ester increase genistein phorbol_ester inhibit treatment-induced increase ATP increase thapsigargin increase t-Bu2BHQ increase Downstream Regulation Effect of modification (function):  enzymatic activity, induced Comments:  The in vitro enzymatic activation produced by ERK-mediated phosphorylation of S31 much smaller than the activation produced by PKA-mediated phosphorylation of S40

S40-p - TH (rat) ▲

Modsite: RFIGRRQsLIEDARK SwissProt Entrez-Gene Orthologous residues TH (human): S71‑p, TH iso2 (human): S67‑p, TH iso3 (human): S40‑p, TH iso4 (human): S44‑p, TH (mouse): S40‑p, TH (rat): S40‑p, TH (cow): S40‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes cAMP_analog increase genistein cAMP_analog no effect upon treatment-induced increase ATP increase Theophylline ATP inhibit treatment-induced increase NGF no change compared to control bradykinin no change compared to control muscarine no change compared to control caffeine no change compared to control thapsigargin no change compared to control t-Bu2BHQ no change compared to control Downstream Regulation Effect of modification (function):  enzymatic activity, induced Comments:  The in vitro enzymatic activation produced by PKA-mediated phosphorylation of S40 much greater than the activation produced by ERK-mediated phosphorylation of S31