Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 28249908
Jansen VM, et al. (2017) Kinome-Wide RNA Interference Screen Reveals a Role for PDK1 in Acquired Resistance to CDK4/6 Inhibition in ER-Positive Breast Cancer. Cancer Res 77, 2488-2499 28249908
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

T308-p - Akt1 (human)
Modsite: kDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  HCC1428 (breast cell), HCC1500 (breast cell), MCF-7 (breast cell), T47D (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GSK2334470 decrease

S477-p - Akt1 (human)
Modsite: PQFsysAsGtA____ SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S477‑p, Akt1 iso2 (human): S415‑p, Akt1 (mouse): S477‑p, Akt1 (rat): S477‑p, Akt1 (fruit fly): T603‑p, Akt1 (cow): S477‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  HCC1428 (breast cell), HCC1500 (breast cell), MCF-7 (breast cell), T47D (breast cell)
Cellular systems studied:  cell lines
Species studied:  human

T479-p - Akt1 (human)
Modsite: FsysAsGtA______ SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T479‑p, Akt1 iso2 (human): T417‑p, Akt1 (mouse): T479‑p, Akt1 (rat): T479‑p, Akt1 (fruit fly): T605‑p, Akt1 (cow): T479‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  HCC1428 (breast cell), HCC1500 (breast cell), MCF-7 (breast cell), T47D (breast cell)
Cellular systems studied:  cell lines
Species studied:  human

S780-p - Rb (human)
Modsite: strPPtLsPIPHIPr SwissProt Entrez-Gene
Orthologous residues
Rb (human): S780‑p, Rb (mouse): S773‑p, Rb (rat): S772‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  HCC1428 (breast cell), HCC1500 (breast cell), MCF-7 (breast cell), T47D (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GSK2334470, ribociclib decrease

S227-p - RSK2 (human)
Modsite: DHEKKAysFCGtVEy SwissProt Entrez-Gene
Orthologous residues
RSK2 (human): S227‑p, RSK2 (mouse): S227‑p, RSK2 (rat): S227‑p, RSK2 (cow): S227‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  HCC1428 (breast cell), HCC1500 (breast cell), MCF-7 (breast cell), T47D (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GSK2334470, ribociclib decrease

S235-p - S6 (human)
Modsite: IAKRRRLssLRAsts SwissProt Entrez-Gene
Orthologous residues
S6 (human): S235‑p, S6 (mouse): S235‑p, S6 (rat): S235‑p, S6 (fruit fly): A234‑p, S6 (cow): S235‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  HCC1428 (breast cell), HCC1500 (breast cell), MCF-7 (breast cell), T47D (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDK1 (human) increase PDK1 siRNA decrease
ribociclib increase
GSK2334470 ribociclib inhibit treatment-induced increase
GSK2334470, ribociclib decrease

S236-p - S6 (human)
Modsite: AKRRRLssLRAstsK SwissProt Entrez-Gene
Orthologous residues
S6 (human): S236‑p, S6 (mouse): S236‑p, S6 (rat): S236‑p, S6 (fruit fly): S235‑p, S6 (cow): S236‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  HCC1428 (breast cell), HCC1500 (breast cell), MCF-7 (breast cell), T47D (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDK1 (human) increase PDK1 siRNA decrease
ribociclib increase
GSK2334470 ribociclib inhibit treatment-induced increase
GSK2334470, ribociclib decrease