Matzinger M, et al. (2020) AMPK leads to phosphorylation of the transcription factor Nrf2, tuning transactivation of selected target genes. Redox Biol 29, 101393 31805502

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

Download

S215-p - NRF2 (human) ▲
Modsite: VETTMVPsPEAKLTE SwissProt Entrez-Gene Orthologous residues NRF2 (human): S215‑p, NRF2 (mouse): S207‑p, NRF2 (rat): S214‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse

S234-p - NRF2 (human) ▲
Modsite: HFYSSIPsMEKEVGN SwissProt Entrez-Gene Orthologous residues NRF2 (human): S234‑p, NRF2 (mouse): S227‑p, NRF2 (rat): S234‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

S243-p - NRF2 (human) ▲
Modsite: EKEVGNCsPHFLNAF SwissProt Entrez-Gene Orthologous residues NRF2 (human): S243‑p, NRF2 (mouse): G236‑p, NRF2 (rat): S243‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse

S374-p - NRF2 (human) ▲

Modsite: GDtLLGLsDsEVEEL SwissProt Entrez-Gene Orthologous residues NRF2 (human): S374‑p, NRF2 (mouse): S365‑p, NRF2 (rat): S372‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE AMPKA1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE AMPKA1 (human) co-immunoprecipitation, genetic knockout/knockin of upstream enzyme, pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes MG132, A-769662 increase compound_C decrease Downstream Regulation Effect of modification (function):  activity, induced, molecular association, regulation, protein stabilization Effect of modification (process):  signaling pathway regulation, transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays BTRC (human) Induces co-immunoprecipitation Comments:  transactivates Nrf2 target genes; prolongs NRF2 abundance;

S376-p - NRF2 (human) ▲
Modsite: tLLGLsDsEVEELDS SwissProt Entrez-Gene Orthologous residues NRF2 (human): S376‑p, NRF2 (mouse): S367‑p, NRF2 (rat): S374‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse

S408-p - NRF2 (human) ▲

Modsite: GDMVQPLsPSQGQST SwissProt Entrez-Gene Orthologous residues NRF2 (human): S408‑p, NRF2 (mouse): S400‑p, NRF2 (rat): S407‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE AMPKA1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE AMPKA1 (human) co-immunoprecipitation, genetic knockout/knockin of upstream enzyme, pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes MG132, A-769662 increase compound_C decrease Downstream Regulation Effect of modification (function):  activity, induced, molecular association, regulation, protein stabilization Effect of modification (process):  signaling pathway regulation, transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays BTRC (human) Induces co-immunoprecipitation Comments:  transactivates Nrf2 target genes; prolongs NRF2 abundance;

T425-p - NRF2 (human) ▲
Modsite: HDAQCENtPEKELPV SwissProt Entrez-Gene Orthologous residues NRF2 (human): T425‑p, NRF2 (mouse): T417‑p, NRF2 (rat): T424‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse

S433-p - NRF2 (human) ▲

Modsite: PEKELPVsPGHrktP SwissProt Entrez-Gene Orthologous residues NRF2 (human): S433‑p, NRF2 (mouse): S425‑p, NRF2 (rat): S432‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE AMPKA1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE AMPKA1 (human) co-immunoprecipitation, genetic knockout/knockin of upstream enzyme, pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes MG132, A-769662 increase compound_C decrease Downstream Regulation Effect of modification (function):  activity, induced, molecular association, regulation, protein stabilization Effect of modification (process):  signaling pathway regulation, transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays BTRC (human) Induces co-immunoprecipitation Comments:  transactivates Nrf2 target genes; prolongs NRF2 abundance;

S577-p - NRF2 (human) ▲
Modsite: DEDGkPysPSEYSLQ SwissProt Entrez-Gene Orthologous residues NRF2 (human): S577‑p, NRF2 (mouse): S569‑p, NRF2 (rat): S576‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse