Curated Information
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Home > Curated Information Page > PubMed Id: 20832730
Gao D, et al. (2010) Rictor forms a complex with Cullin-1 to promote SGK1 ubiquitination and destruction. Mol Cell 39, 797-808 20832730
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical), MEF (fibroblast) [RICTOR (human), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LY294002 decrease
RICTOR (human) increase Rictor shRNA inhibits
insulin increase

T412-p - p70S6K (human)
Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical), MEF (fibroblast) [RICTOR (human), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Akt1 (human) increase Akt1 shRNA inhibits
RICTOR (human) no change compared to control Rictor shRNA no change
Raptor (human) increase Raptor shRNA decreases
mTOR (human) increase mTor shRNA inhibits
insulin increase
LY294002 decrease

T1135-p - RICTOR (human)
Modsite: NRRIRtLtEPsVDFN SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): T1135‑p, RICTOR iso3 (human): T1135‑p, RICTOR iso4 (human): , RICTOR (mouse): T1135‑p, RICTOR (rat): T1120‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical), MEF (fibroblast) [RICTOR (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE p70S6K (human) mutation in upstream enzyme recognition motif, transfection of wild-type enzyme, transfection of inactive enzyme, transfection of constitutively active enzyme
KINASE SGK1 (human) mutation in upstream enzyme recognition motif, transfection of wild-type enzyme, phosphopeptide analysis
KINASE Akt1 (human) phospho-antibody, mutation in upstream enzyme recognition motif, phosphopeptide analysis, transfection of constitutively active enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LY294002 decrease
RICTOR (human) increase Rictor shRNA inhibits
RICTOR (mouse) increase Rictor shRNA inhibits
LY294002 RICTOR (mouse) no effect upon treatment-induced decrease no effect on Rictor shRNA decrease
Akt1 (human) increase Akr1 shRNA inhibits
RICTOR (human) increase Rictor shRNA inhibits
Raptor (human) no change compared to control Raptor shRNA no change
mTOR (human) increase mTor shRNA inhibits
insulin increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation, ubiquitination
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CUL1 (human) Disrupts molecular association, regulation co-immunoprecipitation
Comments:  T1135 phosphorylation reduces Rictor ubiquitination of SGK1.

S422-p - SGK1 (human)
Modsite: AEAFLGFsYAPPTDS SwissProt Entrez-Gene
Orthologous residues
SGK1 (human): S422‑p, SGK1 iso2 (human): S517‑p, SGK1 iso3 (human): S436‑p, SGK1 (mouse): S422‑p, SGK1 (rat): S421‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical), MEF (fibroblast) [RICTOR (human), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
rapamycin decrease
insulin increase slight increase

S473-p - Akt1 (mouse)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical), MEF (fibroblast) [RICTOR (human), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
RICTOR (mouse) increase Rictor -/- decreases
serum RICTOR (mouse) increase Rictor -/- inhibits

T1135-p - RICTOR (mouse)
Modsite: NRRIRtLtEPsVDLN SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): T1135‑p, RICTOR iso3 (human): T1135‑p, RICTOR iso4 (human): , RICTOR (mouse): T1135‑p, RICTOR (rat): T1120‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical), MEF (fibroblast) [RICTOR (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  human