Curated Information
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Home > Curated Information Page > PubMed Id: 20925653
Chiang CW, Liu WK, Chiang CW, Chou CK (2010) Phosphorylation-dependent association of the G4-1/G5PR regulatory subunit with IKKβ negatively modulates NF-κB activation through recruitment of protein phosphatase 5. Biochem J 433, 187-96 20925653
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S181-p - IKKB (mouse)
Modsite: DQGsLCTsFVGTLQy SwissProt Entrez-Gene
Orthologous residues
IKKB (human): S181‑p, IKKB (mouse): S181‑p, IKKB (rat): S181‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKKB (mouse) pharmacological inhibitor of upstream enzyme, transfection of constitutively active enzyme, transfection of dominant-negative enzyme
Comments:  based on a functional assay (interaction with PPP2R3C)
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
wedelolactone decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PPP2R3C (human) Induces pull-down assay
Comments:  interaction with PPP2R3C (aka G4-1) downregulates IKK-beta activity; S181 phosphorylation increased in S679/682/689/692/697/701/705A mutant

S679-p - IKKB (mouse)
Modsite: sPDsMNVsRLsHPGQ SwissProt Entrez-Gene
Orthologous residues
IKKB (human): S679‑p, IKKB (mouse): S679‑p, IKKB (rat): S679‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKKB (mouse) pharmacological inhibitor of upstream enzyme, transfection of constitutively active enzyme, transfection of dominant-negative enzyme
Comments:  based on a functional assay (interaction with PPP2R3C)
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
wedelolactone decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PPP2R3C (human) Induces pull-down assay
Comments:  interaction with PPP2R3C (aka G4-1) downregulates IKK-beta activity; S181 phosphorylation increased in S679/682/689/692/697/701/705A mutant

S682-p - IKKB (mouse)
Modsite: sMNVsRLsHPGQLMs SwissProt Entrez-Gene
Orthologous residues
IKKB (human): S682‑p, IKKB (mouse): S682‑p, IKKB (rat): S682‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKKB (mouse) pharmacological inhibitor of upstream enzyme, transfection of constitutively active enzyme, transfection of dominant-negative enzyme
Comments:  based on a functional assay (interaction with PPP2R3C)
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
wedelolactone decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PPP2R3C (human) Induces pull-down assay
Comments:  interaction with PPP2R3C (aka G4-1) downregulates IKK-beta activity; S181 phosphorylation increased in S679/682/689/692/697/701/705A mutant

S689-p - IKKB (mouse)
Modsite: sHPGQLMsQPsSACD SwissProt Entrez-Gene
Orthologous residues
IKKB (human): S689‑p, IKKB (mouse): S689‑p, IKKB (rat): S689‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKKB (mouse) pharmacological inhibitor of upstream enzyme, transfection of constitutively active enzyme, transfection of dominant-negative enzyme
Comments:  based on a functional assay (interaction with PPP2R3C)
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
wedelolactone decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PPP2R3C (human) Induces pull-down assay
Comments:  interaction with PPP2R3C (aka G4-1) downregulates IKK-beta activity; S181 phosphorylation increased in S679/682/689/692/697/701/705A mutant

S692-p - IKKB (mouse)
Modsite: GQLMsQPsSACDsLP SwissProt Entrez-Gene
Orthologous residues
IKKB (human): S692‑p, IKKB (mouse): S692‑p, IKKB (rat): S692‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKKB (mouse) pharmacological inhibitor of upstream enzyme, transfection of constitutively active enzyme, transfection of dominant-negative enzyme
Comments:  based on a functional assay (interaction with PPP2R3C)
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
wedelolactone decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PPP2R3C (human) Induces pull-down assay
Comments:  interaction with PPP2R3C (aka G4-1) downregulates IKK-beta activity; S181 phosphorylation increased in S679/682/689/692/697/701/705A mutant

S697-p - IKKB (mouse)
Modsite: QPsSACDsLPEsDKK SwissProt Entrez-Gene
Orthologous residues
IKKB (human): S697‑p, IKKB (mouse): S697‑p, IKKB (rat): S697‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKKB (mouse) pharmacological inhibitor of upstream enzyme, transfection of constitutively active enzyme, transfection of dominant-negative enzyme
Comments:  based on a functional assay (interaction with PPP2R3C)
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
wedelolactone decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PPP2R3C (human) Induces pull-down assay
Comments:  interaction with PPP2R3C (aka G4-1) downregulates IKK-beta activity; S181 phosphorylation increased in S679/682/689/692/697/701/705A mutant

S701-p - IKKB (mouse)
Modsite: ACDsLPEsDKKsEEL SwissProt Entrez-Gene
Orthologous residues
IKKB (human): P701‑p, IKKB (mouse): S701‑p, IKKB (rat): S701‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKKB (mouse) pharmacological inhibitor of upstream enzyme, transfection of constitutively active enzyme, transfection of dominant-negative enzyme
Comments:  based on a functional assay (interaction with PPP2R3C)
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
wedelolactone decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PPP2R3C (human) Induces pull-down assay
Comments:  interaction with PPP2R3C (aka G4-1) downregulates IKK-beta activity; S181 phosphorylation increased in S679/682/689/692/697/701/705A mutant

S705-p - IKKB (mouse)
Modsite: LPEsDKKsEELVAEA SwissProt Entrez-Gene
Orthologous residues
IKKB (human): S705‑p, IKKB (mouse): S705‑p, IKKB (rat): S705‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKKB (mouse) pharmacological inhibitor of upstream enzyme, transfection of constitutively active enzyme, transfection of dominant-negative enzyme
Comments:  based on a functional assay (interaction with PPP2R3C)
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
wedelolactone decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PPP2R3C (human) Induces pull-down assay
Comments:  interaction with PPP2R3C (aka G4-1) downregulates IKK-beta activity; S181 phosphorylation increased in S679/682/689/692/697/701/705A mutant