Curated Information
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Home > Curated Information Page > PubMed Id: 20872791
Kai JI, et al. (2010) Glycogen synthase kinase-3β indirectly facilitates interferon-γ-induced nuclear factor-κB activation and nitric oxide biosynthesis. J Cell Biochem 111, 1522-30 20872791
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S536-p - NFkB-p65 (human)
Modsite: sGDEDFSsIADMDFS SwissProt Entrez-Gene
Orthologous residues
NFkB‑p65 (human): S536‑p, NFkB‑p65 (mouse): S534‑p, NFkB‑p65 (rat): S535‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  mononuclear
Cellular systems studied:  primary cells
Species studied:  human
Comments:  peripheral blood mononuclear cells
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IFN-gamma increase
GSK-3_inhibitor_X IFN-gamma inhibit treatment-induced increase

S32-p - IkB-alpha (mouse)
Modsite: LVDDRHDsGLDsMKD SwissProt Entrez-Gene
Orthologous residues
IkB‑alpha (human): S32‑p, IkB‑alpha (mouse): S32‑p, IkB‑alpha (rat): S32‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  RAW 264.7 (macrophage)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IFN-gamma increase
okadaic_acid IFN-gamma inhibit treatment-induced increase
AG17 IFN-gamma inhibit treatment-induced increase
AG490 IFN-gamma inhibit treatment-induced increase
2-AP IFN-gamma inhibit treatment-induced increase

S181-p - IKKB (mouse)
Modsite: DQGsLCTsFVGTLQy SwissProt Entrez-Gene
Orthologous residues
IKKB (human): S181‑p, IKKB (mouse): S181‑p, IKKB (rat): S181‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  RAW 264.7 (macrophage)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IFN-gamma increase
GSK-3_inhibitor_X IFN-gamma inhibit treatment-induced increase
NSC-87877 GSK-3_inhibitor_X, IFN-gamma inhibit treatment-induced decrease

S534-p - NFkB-p65 (mouse)
Modsite: sGDEDFssIADMDFS SwissProt Entrez-Gene
Orthologous residues
NFkB‑p65 (human): S536‑p, NFkB‑p65 (mouse): S534‑p, NFkB‑p65 (rat): S535‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  RAW 264.7 (macrophage)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IFN-gamma increase
GSK-3_inhibitor_X IFN-gamma inhibit treatment-induced increase
IFN-gamma increase
GSK-3_inhibitor_X IFN-gamma inhibit treatment-induced increase
NSC-87877 GSK-3_inhibitor_X, IFN-gamma inhibit treatment-induced decrease

T409-p - PKR (mouse)
Modsite: ENDGKSRtRRTGTLQ SwissProt Entrez-Gene
Orthologous residues
PKR (human): T446‑p, PKR (mouse): T409‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  RAW 264.7 (macrophage)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IFN-gamma increase
2-AP IFN-gamma inhibit treatment-induced increase
AG490 IFN-gamma inhibit treatment-induced increase
GSK-3_inhibitor_X IFN-gamma inhibit treatment-induced increase
NSC-87877 GSK-3_inhibitor_X, IFN-gamma inhibit treatment-induced decrease