Gagnon KB, Delpire E (2010) On the substrate recognition and negative regulation of SPAK, a kinase modulating Na+-K+-2Cl- cotransport activity. Am J Physiol Cell Physiol 299, C614-20 20463172

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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T200-p - NKCC1 (mouse) ▲

Modsite: YYDtHTNtYYLRtFG SwissProt Entrez-Gene Orthologous residues NKCC1 (human): T207‑p, NKCC1 iso3 (human): T207‑p, NKCC1 (mouse): T200‑p, NKCC1 (rat): T198‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  oocyte Cellular systems studied:  primary cells Species studied:  frog Downstream Regulation Effect of modification (function):  activity, induced Comments:  Enzymatic activation by SLK3 requires two threonines separated by 4 amino acids.

T205-p - NKCC1 (mouse) ▲

Modsite: TNtYYLRtFGHNtMD SwissProt Entrez-Gene Orthologous residues NKCC1 (human): T212‑p, NKCC1 iso3 (human): T212‑p, NKCC1 (mouse): T205‑p, NKCC1 (rat): T203‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  oocyte Cellular systems studied:  primary cells Species studied:  frog Downstream Regulation Effect of modification (function):  activity, induced Comments:  Enzymatic activation by SLK3 requires two threonines separated by 4 amino acids.

T243-p - STLK3 (mouse) ▲

Modsite: tRNKVRKtFVGtPCW SwissProt Entrez-Gene Orthologous residues STLK3 (human): T231‑p, STLK3 (mouse): T243‑p, STLK3 (rat): T240‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  oocyte Cellular systems studied:  primary cells Species studied:  frog Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE WNK4 (mouse) mutation in upstream enzyme recognition motif, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  enzymatic activity, induced

S383-p - STLK3 (mouse) ▲

Modsite: VRRVPGssGHLHKTE SwissProt Entrez-Gene Orthologous residues STLK3 (human): S371‑p, STLK3 (mouse): S383‑p, STLK3 (rat): S380‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  oocyte Cellular systems studied:  primary cells Species studied:  frog Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE WNK4 (mouse) mutation in upstream enzyme recognition motif, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  enzymatic activity, induced