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Home > Curated Information Page > PubMed Id: 21045017
Wang P, et al. (2011) Serine phosphorylation of NPM-ALK, which is dependent on the auto-activation of the kinase activation loop, contributes to its oncogenic potential. Carcinogenesis 32, 146-53 21045017
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T202-p - ERK1 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293GP (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NPM-ALK (human) increase

Y204-p - ERK1 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293GP (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NPM-ALK (human) increase

T185-p - ERK2 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293GP (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NPM-ALK (human) increase

Y187-p - ERK2 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293GP (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NPM-ALK (human) increase

T183-p - JNK1 (human)
Modsite: AGtsFMMtPyVVtRY SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293GP (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NPM-ALK (human) increase

Y185-p - JNK1 (human)
Modsite: tsFMMtPyVVtRYYR SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293GP (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NPM-ALK (human) increase

S135-p - NPM-ALK (human)
Modsite: AMQMELQsPEyKLSK GenPept
Orthologous residues
NPM‑ALK (human): S135‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  lymphoma, anaplastic large cell lymphoma, T cell lymphoma
Relevant cell lines - cell types - tissues:  293GP (epithelial), Karpas-299 (T lymphocyte), SU-DHL1 (T lymphocyte), SUP-M2 (T lymphocyte)
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PD98059 decrease
SB203580 decrease
U0126 decrease
SP600125 decrease
Downstream Regulation
Effect of modification (function):  phosphorylation
Effect of modification (process):  carcinogenesis, altered, cell growth, altered
Comments:  mutation of this site reduces the phosphorylation of JNK, ERK1/2, Stat6, required for phosphorylation of residues S164 and S497

Y138-p - NPM-ALK (human)
Modsite: MELQsPEyKLSKLRT GenPept
Orthologous residues
NPM‑ALK (human): Y138‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293GP (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

Y152-p - NPM-ALK (human)
Modsite: TSTIMTDyNPNyCFA GenPept
Orthologous residues
NPM‑ALK (human): Y152‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293GP (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

Y156-p - NPM-ALK (human)
Modsite: MTDyNPNyCFAGKTS GenPept
Orthologous residues
NPM‑ALK (human): Y156‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293GP (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S164-p - NPM-ALK (human)
Modsite: CFAGKTSsISDLKEV GenPept
Orthologous residues
NPM‑ALK (human): S164‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  lymphoma, anaplastic large cell lymphoma, T cell lymphoma
Relevant cell lines - cell types - tissues:  293GP (epithelial), Karpas-299 (T lymphocyte), SU-DHL1 (T lymphocyte), SUP-M2 (T lymphocyte)
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
D4476 decrease
GSK-3_inhibitor_IX decrease
Downstream Regulation
Effect of modification (function):  phosphorylation
Effect of modification (process):  carcinogenesis, altered, cell growth, altered
Comments:  mutation of this site reduces the phosphorylation of JNK, ERK1/2, Stat6

Y191-p - NPM-ALK (human)
Modsite: HGAFGEVyEGQVSGM GenPept
Orthologous residues
NPM‑ALK (human): Y191‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293GP (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

Y338-p - NPM-ALK (human)
Modsite: FGMARDIyRAsyyRK GenPept
Orthologous residues
NPM‑ALK (human): Y338‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293GP (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

Y342-p - NPM-ALK (human)
Modsite: RDIyRAsyyRKGGCA GenPept
Orthologous residues
NPM‑ALK (human): Y342‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293GP (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

Y343-p - NPM-ALK (human)
Modsite: DIyRAsyyRKGGCAM GenPept
Orthologous residues
NPM‑ALK (human): Y343‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293GP (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

Y419-p - NPM-ALK (human)
Modsite: KNCPGPVyRIMTQCW GenPept
Orthologous residues
NPM‑ALK (human): Y419‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293GP (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S497-p - NPM-ALK (human)
Modsite: AKREEERsPAAPPPL GenPept
Orthologous residues
NPM‑ALK (human): S497‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  lymphoma, anaplastic large cell lymphoma, T cell lymphoma
Relevant cell lines - cell types - tissues:  293GP (epithelial), Karpas-299 (T lymphocyte), SU-DHL1 (T lymphocyte), SUP-M2 (T lymphocyte)
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PD98059 decrease
SB203580 decrease
U0126 decrease
SP600125 decrease
Downstream Regulation
Effect of modification (function):  phosphorylation
Effect of modification (process):  carcinogenesis, altered, cell growth, altered
Comments:  mutation of this site reduces the phosphorylation of JNK, ERK1/2, Stat6

Y567-p - NPM-ALK (human)
Modsite: TSLWNPTyGSWFTEK GenPept
Orthologous residues
NPM‑ALK (human): Y567‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293GP (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

Y644-p - NPM-ALK (human)
Modsite: FPCGNVNyGyQQQGL GenPept
Orthologous residues
NPM‑ALK (human): Y644‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293GP (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

Y664-p - NPM-ALK (human)
Modsite: TAPGAGHyEDTILKS GenPept
Orthologous residues
NPM‑ALK (human): Y664‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293GP (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

Y641-p - STAT6 (human)
Modsite: MGkDGRGyVPAtIkM SwissProt Entrez-Gene
Orthologous residues
STAT6 (human): Y641‑p, STAT6 iso2 (human): Y467‑p, STAT6 (mouse): Y641‑p, STAT6 (rat): Y641‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293GP (epithelial), Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NPM-ALK (human) increase