Curated Information
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Home > Curated Information Page > PubMed Id: 31677786
Cariulo C, et al. (2019) Ultrasensitive quantitative measurement of huntingtin phosphorylation at residue S13. Biochem Biophys Res Commun 31677786
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T3-p - Huntingtin (human)
Modsite: _____MAtLEkLMkA SwissProt Entrez-Gene
Orthologous residues
Huntingtin (human): T3‑p, Huntingtin (mouse): T3‑p, Huntingtin (rat):

S13-p - Huntingtin (human)
Modsite: kLMkAFEsLksFQQQ SwissProt Entrez-Gene
Orthologous residues
Huntingtin (human): S13‑p, Huntingtin (mouse): S13‑p, Huntingtin (rat): S6‑p
Characterization
Methods used to characterize site in vivo immunoassay, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  Huntington's disease
Relevant cell lines - cell types - tissues:  'neuron, cortical', 'neuron, striatal', brain, HEK293T (epithelial), STHdh ('neuron, striatal')
Cellular systems studied:  cell lines, primary cells, tissue
Species studied:  human, mouse

S16-p - Huntingtin (human)
Modsite: kAFEsLksFQQQQQQ SwissProt Entrez-Gene
Orthologous residues
Huntingtin (human): S16‑p, Huntingtin (mouse): S16‑p, Huntingtin (rat): S9‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

T3-p - Huntingtin (mouse)
Modsite: _____MAtLEkLMkA SwissProt Entrez-Gene
Orthologous residues
Huntingtin (human): T3‑p, Huntingtin (mouse): T3‑p, Huntingtin (rat):

S13-p - Huntingtin (mouse)
Modsite: kLMkAFEsLksFQQQ SwissProt Entrez-Gene
Orthologous residues
Huntingtin (human): S13‑p, Huntingtin (mouse): S13‑p, Huntingtin (rat): S6‑p
Characterization
Methods used to characterize site in vivo immunoassay, phospho-antibody, western blotting
Disease tissue studied:  Huntington's disease
Relevant cell lines - cell types - tissues:  'neuron, cortical', 'neuron, striatal', brain, HEK293T (epithelial), STHdh ('neuron, striatal')
Cellular systems studied:  cell lines, primary cells, tissue
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ganglioside_GM1 increase in StHdh Q111/Q111 cells

S16-p - Huntingtin (mouse)
Modsite: kAFEsLksFQQQQQQ SwissProt Entrez-Gene
Orthologous residues
Huntingtin (human): S16‑p, Huntingtin (mouse): S16‑p, Huntingtin (rat): S9‑p