Curated Information
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Home > Curated Information Page > PubMed Id: 18083840
Kang J, Chen Y, Zhao Y, Yu H (2007) Autophosphorylation-dependent activation of human Mps1 is required for the spindle checkpoint. Proc Natl Acad Sci U S A 104, 20232-7 18083840
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S281-p - TTK (human)
Modsite: VPVNLLNsPDCDVkt SwissProt Entrez-Gene
Orthologous residues
TTK (human): S281‑p, TTK iso2 (human): S281‑p, TTK (mouse): S277‑p, TTK iso2 (mouse): S277‑p, TTK (rat): S277‑p, TTK (frog): S283‑p

T360-p - TTK (human)
Modsite: sITLKNktEssLLAk SwissProt Entrez-Gene
Orthologous residues
TTK (human): T360‑p, TTK iso2 (human): T360‑p, TTK (mouse): T380‑p, TTK iso2 (mouse): T354‑p, TTK (rat): T355‑p, TTK (frog): Q388‑p

S363-p - TTK (human)
Modsite: LKNktEssLLAkLEE SwissProt Entrez-Gene
Orthologous residues
TTK (human): S363‑p, TTK iso2 (human): S363‑p, TTK (mouse): S383‑p, TTK iso2 (mouse): S357‑p, TTK (rat): S358‑p, TTK (frog): S391‑p

T371-p - TTK (human)
Modsite: LLAkLEEtkEyQEPE SwissProt Entrez-Gene
Orthologous residues
TTK (human): T371‑p, TTK iso2 (human): T371‑p, TTK (mouse): T390‑p, TTK iso2 (mouse): T364‑p, TTK (rat): P365‑p, TTK (frog): P393‑p

S382-p - TTK (human)
Modsite: QEPEVPEsNQkQWQS SwissProt Entrez-Gene
Orthologous residues
TTK (human): S382‑p, TTK iso2 (human): S382‑p, TTK (mouse): R397‑p, TTK iso2 (mouse): R371‑p, TTK (rat): R376‑p, TTK (frog): I404‑p

S436-p - TTK (human)
Modsite: VFSVskQsPPIsTSk SwissProt Entrez-Gene
Orthologous residues
TTK (human): S436‑p, TTK iso2 (human): S435‑p, TTK (mouse): S435‑p, TTK iso2 (mouse): S409‑p, TTK (rat): S414‑p, TTK (frog): S459‑p

T453-p - TTK (human)
Modsite: DPkSICktPsSNtLD SwissProt Entrez-Gene
Orthologous residues
TTK (human): T453‑p, TTK iso2 (human): T452‑p, TTK (mouse): T452‑p, TTK iso2 (mouse): T426‑p, TTK (rat): T431‑p, TTK (frog): T476‑p

T468-p - TTK (human)
Modsite: DyMsCFrtPVVkNDF SwissProt Entrez-Gene
Orthologous residues
TTK (human): T468‑p, TTK iso2 (human): T467‑p, TTK (mouse): T467‑p, TTK iso2 (mouse): T441‑p, TTK (rat): T446‑p, TTK (frog): T491‑p

T676-p - TTK (human)
Modsite: NQMQPDttsVVkDSQ SwissProt Entrez-Gene
Orthologous residues
TTK (human): T676‑p, TTK iso2 (human): T675‑p, TTK (mouse): T675‑p, TTK iso2 (mouse): T649‑p, TTK (rat): T654‑p, TTK (frog): T697‑p
Characterization
Methods used to characterize site in vivo 2D analysis, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)
Comments:  transactivation in vitro
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
AP20187 increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, molecular association, regulation
Effect of modification (process):  cell cycle regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
TTK (human) Not reported
Comments:  T676 phosphorylation required for proper spindle checkpoint signaling; dimerization of TTK induces its kinase activity and autophosphorylation

S821-p - TTK (human)
Modsite: GQLVGLNsPNsILkA SwissProt Entrez-Gene
Orthologous residues
TTK (human): S821‑p, TTK iso2 (human): S820‑p, TTK (mouse): S820‑p, TTK iso2 (mouse): S794‑p, TTK (rat): S799‑p, TTK (frog): S844‑p